胰岛素联合硫酸镁对大鼠局灶性脑缺血的保护作用

目的观察胰岛素联合硫酸镁对大鼠局灶性脑缺血的保护作用及联合用药的优势。方法将30只Wistar大鼠随机分为5组,即空白对照组、假手术组、胰岛素组、硫酸镁组和联合用药组,每组6只。除假手术组外,其余5组采用Longa线栓法建立局灶性脑缺血模型。空白对照组术后腹腔注射0.9%氯化钠注射液2 mL;假手术组不采用药物干预;胰岛素组术后予胰岛素2 U/kg腹腔注射;硫酸镁组予硫酸镁500 mg/kg腹腔注射;联合用药组予胰岛素2 U/kg和硫酸镁500 mg/kg腹腔注射。测定血糖及血镁浓度,进行神经功能评分,计算梗死体积。结果空白对照组、假手术组术后血糖升高,与术前比较差异均有统计学意义（P〈0.05）,联合用药组术后30 min、2 h血糖均较空白对照组降低（P〈0.05）;空白对照组血镁低于假手术组（P〈0.05）,硫酸镁组、联合用药组血镁高于空白对照组（P〈0.05）;假手术组梗死体积为0,胰岛素组、硫酸镁组及联合用药组梗死体积均较空白对照组减小（P〈0.05）,联合用药组的梗死体积又较胰岛素组、硫酸镁组明显降低（P〈0.05）;假手术组神经功能评分为0分,胰岛素组、硫酸镁组和联合用药组术后6、24 h神经功能评分均较空白对照组低（P〈0.05）,联合用药组又较胰岛素组、硫酸镁组为低（P〈0.05）。结论胰岛素联合硫酸镁对大鼠局灶性脑缺血有保护作用,优于单独应用胰岛素或硫酸镁。

Protection of combination of insulin and magnesium sulfate in rat with focal cerebral ischemia

Objective To investigate the effect of combination therapy with insulin and magnesium sulfate on the middle cerebral artery occlusion in rat and compare this effect with that of monotherapy of insulin or magnesium sulfate.Methods Thirty male Wistar rats were randomly divided into five groups： control group,sham operation group,insulin group,magnesium sulfate group;insulin plus magnesium sulfate group.Model rats with focal cerebral ischemia were established by Longa suture method.After the operation,insulin and/or magnesium sulfate were given to the animals in each group. Then the blood glucose and magnesium concentration, the volume of infarction and the neurofunction deficiency were detected. Results （1）The blood glucose concentration in control group and sham operation group in 30 minutes and 2 hours after operation were higher than that of preoperation （ P 〈0.05 ）. And that of control group in 30 minutes and 2 hours after operation were higher than sham operation group （ P 〈 0.05 ） ; The blood glucose concentration of insulin group and combination therapy group in 30 minutes and 2 hours after operation were not increased compared to that of preoperation. （2） The magnesium concentration of control group was lower than sham operation group（ P 〈 0.05 ） ; The magnesium concentration of magnesium group and combination therapy group were higher than that of control group （ P 〈 0.05 ）. （3） The sham operation group had no infarction ; The infarction volume of insulin group, magnesium group and combination therapy group were decrease compare to control group （ P 〈 0.05 ） ; Furthermore, the infarction volume of combination therapy group was the smallest among all of the treatment groups. （4） The sham operation group had no neurofunction deficiency; The neurofunetion deficiency of insulin group, magnesium group and combination therapy group after operation were lower than that of control group （ P 〈 0. 05 ）. Cerebral ischemia can cause blood glucose increasing due to