| 采用微卫星标记法对白癜风易感基因的初步筛查研究 |
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| 引用本文： | 邱梦桃,余鹏,陈文静,谷梅. 采用微卫星标记法对白癜风易感基因的初步筛查研究[J]. 岭南皮肤性病科杂志, 2011, 18(4): 234-236 |
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| 作者姓名： | 邱梦桃 余鹏 陈文静 谷梅 |
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| 作者单位： | 广东省皮肤性病防治中心,广东,广州,510500 |
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| 摘 要： | 目的：对收集的白癜风38家系183人进行基因精细定位，以期识别白癜风的致病基因。方法：收集白癜风患者及其家系成员的临床资料及血液样本，抽提外周血基因组DNA，选用荧光标记引物及微卫星标记，用Genescan和Genotyper软件进行基因分型，用Linkage软件包进行连锁分析，明确致病基因的区域，并对22q12进行精细定位。结果：发现白癜风致病基因与微卫星标记D22s1163处获得最大连锁值（NPL=2．22，P=0．008，HLOD=1．64，α=53％）。结论：染色体22q12上可能存在广东汉族人的白癌风易戚基呙．
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| 关 键 词： | 白癜风 序列分析 DNA 染色体 连锁 |
Primary Screening Study for Susceptibility Genes of Vitiligo Using Microsatellite Marker Technology |
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| QIU Meng-tao,YU Peng,CHEN Wen-jing,GU Mei. Primary Screening Study for Susceptibility Genes of Vitiligo Using Microsatellite Marker Technology[J]. Southern China Journal of Dermato-Venereology, 2011, 18(4): 234-236 |
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| Authors: | QIU Meng-tao YU Peng CHEN Wen-jing GU Mei |
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| Affiliation: | QIU Meng-tao,YU Peng,CHEN Wen-jing,GU Mei(Guangdong Provincial Centre for Skin Diseases and STIs Control and Prevention,Guangzhou 510500,China) |
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| Abstract: | Objective:To identify pathogenic genes of Vitiligo in 38 Chinese families including a total of 183 individuals in Guangdong province. Methods:The clinical data and the peripheral blood samples of the pedigree members were collected.The genomic DNA was extracted from the blood cells.A genome-wide scan was performed using primers labeled with fluorescence.The primers were designed for human autosomes.The results of sequencing and microsatellite markers were analyzed by the software of Genescan and Genotyper.L... |
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| Keywords: | Vitiligo Sequence analysis DNA Chromosome Linkage genetics |
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