| 重铬酸钾致N-ras基因DNA损伤作用研究 |
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| 引用本文： | 饶朝龙,衡正昌. 重铬酸钾致N-ras基因DNA损伤作用研究[J]. 卫生研究, 2005, 34(3): 297-299 |
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| 作者姓名： | 饶朝龙 衡正昌 |
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| 作者单位： | 四川大学华西公共卫生学院环境卫生教研室,成都,610041 |
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| 基金项目： | 国家自然科学基金资助项目 (No .30 0 70 648) |
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| 摘 要： | 目的 研究重铬酸钾对N -ras基因的DNA损伤作用,探讨其致癌作用分子机制。方法 制备N -ras基因外显子1单链探针;重铬酸钾染毒细胞提取基因组DNA ,再经RDPCR扩增后与探针杂交显色。结果 重铬酸钾染毒剂量10 0 μmol L可检测到DNA损伤片段杂交条带,其损伤片段长于完整的N ras基因外显子1;更高剂量(10 0 0 μmol L)未能检测出DNA损伤作用。结论 重铬酸钾可能造成N- ras基因外显子1上游序列DNA损伤,且该损伤作用可能正是其致癌作用分子机制的关键所在。
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| 关 键 词： | N-ras基因 DNA损伤 重铬酸钾 依赖随机化末端连接物PCR |
| 文章编号： | 1000-8020(2005)03-0297-03 |
| 修稿时间： | 2004-11-12 |
Detecting the DNA damage of N-ras gene induced by potassium dichromate |
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| Rao Chao-long,Heng Zheng-chang. Detecting the DNA damage of N-ras gene induced by potassium dichromate[J]. Journal of hygiene research, 2005, 34(3): 297-299 |
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| Authors: | Rao Chao-long Heng Zheng-chang |
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| Affiliation: | Department of Environmental Health, School of Public Health, Sichuan University, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: Applying RDPCR to detect the DNA damage of N-ras gene induced by Potassium dichromate METHODS: Preparing single-stranded probes of exon 1 of N-ras gene in human. The genomic DNA was treated with Potassium dichromate, then amplified by RDPCR and detected by Southern hybridization with the probe. RESULTS: The clear hybridized bands can be seen in the position which is induced by Potassium dichromate on the dose of 100 micromol/L, but can't be detected on the dose over 1000 mol/L CONCLUSION: It indicates that Potassium dichromate can cause the DNA damage of N-ras gene, which should be the key point of its carcinogenesis mechanisms. |
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| Keywords: | N-ras gene DNA damage potassium dichromate RDPCR |
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