紫外分光光度法测定金莲花口服液含量

目的建立金莲花口服液中黄酮类物质的含量测定方法。方法采用紫外分光光度法,通过紫外扫描确定最大吸收波长,在最大吸收波长处以芦丁为对照品测量吸光度制定标准曲线,并进行样品溶液稳定性试验、重现性试验、精密度试验、回收率试验等。结果精密度试验所测得的吸光度分别为:0.235、0.234、0.234、0.234、0.233,相对标准偏差(RSD)=0.30%,数据表明该试验具有良好的精密度;稳定性试验测定在0、5、10、15、20、25min时吸光度分别为0.249、0.249、0.248、0.245、0.245、0.244,RSD=0.83%,数据表明在25min内对待测物进行吸光度的测定是稳定的;重现性试验在500nm波长处测定吸光度为:0.412、0.411、0.413、0.407、0.407,RSD=0.69%,表示重现性良好;回收率试验测定的平均回收率为98.5%,RSD=0.56%;样品含量的测定测定的3种批号样本的平均含量分别为0.334mg/ml、0.412mg/ml、0.375mg/ml。结论紫外分光光度法简单方便、准确可靠,可以用来测定金莲花口服液中黄酮类物质的含量。

UV spectrophotometry Nasturtium determination of content of oral trollius

Objective To establish the Determination of content of oral trollius.Methods Using UV spectrophotometry,by UV scanning to determine the maximum absorption wavelength at the maximum absorption wavelength of rutin as reference substance punishable by measuring the absorbance standard curve,and the sample solution stability test,test reproducibility,precision testing,and recovery were performed.Results The precision of the measured absorbance： 0.235,0.234,0.234,0.234,0.233;the relative standard deviation（RSD）=0.30%,the data show that the test had good precision;stability test absorbance was measured ： 0.249,0.249,0.248,0.245,0.245,0.244.RSD=0.83%,the data show that within 25min,the treatment was stable to mesure the absorbance of material;absorbance measured by reproduction test was at 500nm wavelength was：0.412,0.411,0.413,0.407,0.407;RSD=0.69%,indicating good reproducibility;recovery test determined the average recovery was 98.5%,RSD=0.56%;the average contents of three batches samples were 0.334mg/ml,0.412mg/ml,and 0.375mg/ml.Conclusion UV spectrophotometry is simple and convenient,accurate and reliable to be used to determine the flavonoids content of oral trollius..