骨髓源性多巴胺前体细胞移植治疗帕金森病模型鼠

目的 探讨骨髓基质干细胞(BMSCs)诱导分化为多巴胺(DA)能前体细胞后移植治疗帕金森病(PD)模型鼠的疗效及作用机制.方法 将纯化的BMSCs诱导为DA前体细胞,免疫荧光染色和流式细胞仪检测TH阳性细胞的表达及百分比.将PD大鼠模型随机分为3组,分别注入诱导、未诱导的BMSCs和生理盐水于模型鼠右侧纹状体区.干预后观察行为学的变化,免疫荧光鉴定以及高效液相色谱检测腑内DA含量的变化.结果 诱导后可见TH阳性细胞,且比例可达10.3%.移植治疗6周后诱导组旋转次数变化多于未诱导组(P<0.05)和生理盐水组(P<0.01);诱导组可见BrdU/TH、BrdU/NSE双标阳性的细胞,未诱导组只见BrdU/GFAP双标细胞;BMSCs诱导组DA脑内含量明显高于未诱导组和生理盐水组(P<0.05),而后两组差异无统计学意义(P>0.05).结论 BMSCs诱导为DA前体细胞移植治疗PD模型的疗效总体好于未诱导的BMSCs组和生理盐水组.

Transplantation of bone marrow-derived stromal cells into DA in vitro for rat models of Parkin-son's disease

Objective To study curative effects and mechanism of bone marrow stromal cells (BMSCs) transplantation on rat models of Parkinson's disease (PD). Methods BMSCs were induced into TH-positive cells, which were stained by immunofluorescence with TH and detected by flow cytometry. The 6-hydroxydopamine (6-OHDA) induced hemiparkinsonian rats were selected and allocated randomly into 3 greups,which were. BMSCs labeled with brdu induced into TH positive cells (1.2×106 cells/each model), BMSCs without induced and physiological saline (12 μl) were injected into right striatum of PD rat respectively. The rotation test was taken at 6 week after transplantation. Brain frozen sections were car-fled out immunofluorescence double staining (BrdU/GFAP,BrdU/NSE and BrdU/TH) at 6 week of BM-SCs. Meanwhile the level of dopamine in nigrostriatal system were observed by High-performance liquid chromatography. Results The percentage of TH-positive cells was 10.3% by detecting with flow cytome-try. Frequency of changed rotation were significantly more in induced than non-induced BMSCs group and normal saline group at 6 week after transplantation. Some BrdU/NSE, BrdU/TH positive cells could be found in the implanted region of BMSCs induction group at 6 week after transplantation. At this time, Br-dU/NSE and BrdU/TH positive cells are not found in the implanted region of BMSCs group but there are BrdU/GFAP positive cells, the level of dopamine in nigrostriatal system in the implanted region of BMSCs induction group at 6 week after transplantation, were more than BMSCs group observed by High-perform-ance liquid chromatography. Conclusion BMSCs induced into TH-positive cells had better effects on treating PD rat models than that of BMSCs without induced group, and physiological saline group. The reasearch provicled the evideme of treating PD.