Effects of Poloxamer 188 on red blood cell membrane properties in sickle cell anaemia |
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| Authors: | Claudine Lapoumeroulie Miklos Rabaï Sophie D. Lefevre Nathalie Lemonne Wassim El Nemer Anaïs Mozar Olivier Français Bruno Le Pioufle Caroline Le Van Kim |
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| Affiliation: | 1. Inserm UMR_S1134, Paris, France;2. Institut National de la Transfusion Sanguine, Paris, France;3. Laboratoire d'Excellence GR‐Ex, Paris, France;4. University of Pecs Medical School, Pecs, Hungary;5. Université Paris Diderot, Sorbonne Paris Cité, Paris, France;6. CHU de Pointe‐à‐Pitre, Pointe‐à‐Pitre, Guadeloupe;7. Inserm, Université des Antilles et de la Guyane, Pointe‐à‐Pitre, Guadeloupe;8. Ecole Normale Supérieure de Cachan, CNRS, BIOMIS‐SATIE, UMR 8029, Cachan, France |
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| Abstract: | Vaso‐occlusive crisis (VOC) is the main acute complication in sickle cell anaemia (SS) and several clinical trials are investigating different drugs to improve the clinical severity of SS patients. A phase III study is currently exploring the profit of Velopoloxamer in SS during VOCs. We analysed, in‐vitro, the effect of poloxamer (P188) on red blood cell (RBC) properties by investigating haemorheology, mechanical and adhesion functions using ektacytometry, microfluidics and dynamic adhesion approaches, respectively. We show that poloxamer significantly reduces blood viscosity, RBC aggregation and adhesion to endothelial cells, supporting the beneficial use of this molecule in SS therapy. |
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| Keywords: | sickle cell anaemia poloxamer red blood cell |
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