| 肺癌p16、Rb基因mRNA和蛋白水平表达的定位观察 |
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| 引用本文： | 苏长青,叶玉坤,汪栋,曹祥荣,单祥年. 肺癌p16、Rb基因mRNA和蛋白水平表达的定位观察[J]. 解放军医学杂志, 2001, 26(2): 85-87,F003 |
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| 作者姓名： | 苏长青 叶玉坤 汪栋 曹祥荣 单祥年 |
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| 作者单位： | 1. 解放军第81医院
2. 南京师范大学生命科学院 |
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| 基金项目： | 国家“九五”重点科技攻关项目资助课题!(编号 96 90 6 0 1 18)，国家自然科学基金资助课题!(编号 3 9670 714 ) |
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| 摘 要： | 为研究p16和Rb基因在蛋白水平和mRNA水平的表达及其肺癌发生发展的关系，采用免疫组化和原位杂交的方法，对89例肺癌手术标本进行了p16和Rb基因表达的定位观察。发现p16基因蛋白的丢失主要发生于非小 细胞肺癌，Rb基因蛋白的丢失主要发生小于细胞肺癌。两种基因各自在蛋白水平和mRNA水平的表达基本相符。提示p16和Rb基因的表达与肺癌的组织学类型密切相关，有可能作为非小细胞肺癌与小细胞肺癌基因分型诊断的分子生物学指标之一；在翻译水平上也存在着引起基因失活的可能机制。
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| 关 键 词： | 肺肿瘤 p16基因 Rb基因 mRNA 免疫组织化学 原位杂交 |
EXPRESSION OF THE p16 AND Rb GENES mRNA AND PROTEIN LEVELS IN LUNG CANCER |
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| Su Changqing,Ye Yukun,Wang Dong et al. EXPRESSION OF THE p16 AND Rb GENES mRNA AND PROTEIN LEVELS IN LUNG CANCER[J]. Medical Journal of Chinese People's Liberation Army, 2001, 26(2): 85-87,F003 |
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| Authors: | Su Changqing Ye Yukun Wang Dong et al |
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| Affiliation: | Su Changqing,Ye Yukun,Wang Dong et al. The 81st Hospital of PLA,Nanjing 210002 |
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| Abstract: | The authors used immunohistochemistry and in situ hybridization to observe the expression of the p16 and Rb mRNA and protein levels in 89 cases of lung cancer. The results showed that the loss of p16 gene expression took place mainly in non small cell lung cancer, and loss of Rb gene expression mainly in small cell lung cancer. The expression of these genes on protein level fundamentally corresponded with mRNA level. It is suggested that the expression of these two genes are related to the histological type of lung cancer, and the p16 and Rb genes can be considered as one of molecular biological targets for gene classification diagnosis. There may exist a mechanism at gene translation level which leads to the inactivation of the p16 and Rb genes. |
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| Keywords: | lung neoplasm p16 gene Rb gene |
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