A better method for confirming <Emphasis Type="Italic">Helicobacter pylori</Emphasis> infection in Mongolian gerbils |
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Authors: | Chao-Hung Kuo Huang-Ming Hu Pei-Yun Tsai Sheau-Fang Yang Lin-Li Chang Jaw-Yuan Wang Angela Chen Chang-Ming Jan Wen-Ming Wang Deng-Chyang Wu |
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Institution: | (1) Division of Internal Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan;(2) Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-You 1st Road, Kaohsiung, 807, Taiwan;(3) Department of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;(4) Department of Nursing, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan;(5) Department of Pathology, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan;(6) Department of Microbiology, Kaohsiung Medical University, Kaohsiung, Taiwan;(7) Division of Gastrointestinal Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;(8) Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan;(9) National Sun Yat-Sen University-Kaohsiung Medical University Joint Center, Kaohsiung, Taiwan |
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Abstract: | Background. Our aim was to evaluate the accuracy of the stool antigen test and the optimal time point for detecting Helicobacter pylori infection in a Mongolian gerbil model. Methods. We inoculated 8-week-old Mongolian gerbils with H. pylori (Vac A (+)/CagA(+)). The gerbil-infected model was developed as follows: H. pylori was put into broth (about 109 CFU/ml), and 50 gerbils were then fed with 1 ml intragastrically twice within a 3-day interval. Another ten gerbils were
fed broth only. Twenty-six weeks after the inoculation, the gerbils were killed. The gastric mucosa was sampled for a series
of examinations including culture, histology, rapid urease test, and polymerase chain reaction. Stool samples for a stool
antigen test, H. pylori-specific stool antigen assay (HpSA), were collected during weeks 4, 6, 8, 12, and 26 after inoculation. Of the 50 gerbils
inoculated with H. pylori, the inoculation was successful in 88%. Severe active gastritis, ulceration, and intestinal metaplasia were obvious. Results. The HpSA test results were sensitivity, 88.6%; specificity, 100%; positive predictive value (PPV), 100%; negative predictive
value (NPV), 54.5%, and accuracy, 90%. The HpSA test began to be more sensitive and accurate (P < 0.05) beginning during week 6 after inoculation. We also found that H. pylori could be detected earlier and more easily in the group with high H. pylori density. Conclusions. HpSA seems to be suitable for confirming colonization of gerbils with H. pylori. The optimal testing time point is around 6 weeks after inoculation. This test is a good choice for long-term observation
of H. pylori infection in Mongolian gerbils. |
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Keywords: | Helicobacter pylori Mongolian gerbil stool antigen test |
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