Effects of growth hormone on insulin-like growth factors and binding proteins before and after renal transplantation |
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Authors: | Margaret J van Renen Sabine Harrer Ken F Jureidini Anne A Martin |
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Institution: | (1) Department of Nephrology, Women’s and Children’s Hospital, North Adelaide, South Australia, 5006, Australia, AU;(2) Child Health Research Institute and Co-operative Research Centre for Tissue Growth and Repair, North Adelaide, South Australia, 5006, Australia, AU |
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Abstract: | A
bstract. Three short children with severe chronic renal failure were treated with recombinant human growth hormone (rhGH) for 2 years.
Each received a transplant in the 2nd year. Serum collected before and during rhGH therapy was analysed retrospectively by
Western ligand blot and immunoblotting techniques. In addition, radioimmunoassays for insulin-like growth factor-I (IGF-I),
IGF binding protein-3 (IGFBP-3), acid-labile subunit (ALS) and IGFBP-1 were performed. IGFBPs in serum, detected by Western
ligand blot, were identified as IGFBP-3, -2, -1 and -4 by immunoblot. The serum concentration of IGF-I in each child rose
approximately fourfold with rhGH before transplantation and subsequently remained elevated. IGFBP-3 levels rose to double
the pretreatment value, but dropped to normal levels following transplantation, while ALS rose with rhGH treatment and remained
increased after transplantation. IGFBP-1 levels changed little with rhGH but fell following transplantation. A low molecular
weight form of IGFBP-3 was noted at 30 kilodaltons on immunoblot which was not clearly seen on the ligand blot. IGFBP-2 was
present as a distinct band on Western ligand blot before transplantation and appeared decreased in intensity subsequently.
IGFBP-1, seen on immunoblot clearly before transplant, disappeared after the transplant. rhGH successfully improved growth
in these children, in association with a fourfold increase in IGF-I levels, which was maintained following transplantation.
The reduction in IGFBPs following transplantation suggests correction of impaired clearance by the diseased kidney.
Received September 5, 1994; received in revised form November 8, 1995; accepted November 21, 1995 |
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Keywords: | : Chronic renal failure Short stature Recombinant human growth hormone Insulin-like growth factors Insulin-like growth factor binding proteins Acid-labile subunit |
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