大鼠溺死时肺组织水通道蛋白1的变化

目的 观察溺死大鼠肺组织中水通道蛋白1(aquaporin AQF1)的变化。方法 取对照组、实验组大鼠肺组织，用免疫组织化学法和免疫电镜胶体金法，观察及定位肺组织中水通道蛋白1。结果 免疫组化显示溺死时肺间质的毛细血管内皮、支气管上皮及肺泡上皮AQP1的阳性表达，积分光密度值差别有统计学意义。免疫电镜显示肺泡(型上皮细胞AQP1的胶体金数的差别有统计学意义。结论 AQP1在大鼠溺死时肺组织中表达有阳性意义且定位在Ⅰ型上皮细胞膜上。

The Changes of Water Channel Protein 1 in the Lungs of the Drown Rat

OBJECTIVE: To observe water channel 1 protein in the lungs of the drown rat. METHODS: Immunohistochemical method and Colloidal gold of the electron microscopy were used to observe the location of aquaporin1(AQP1) on the rat lung tissue between the experiment group and control group. RESULTS: The positive expression of AQP1 was seen in the capillary endotheliocyte of the interstitium and around bronchi and in the alveolar endothelial cells. The difference in the value of integrated optical density between the two groups was statistically significant. AQP1 was located in the type I alveolar cells, and the difference in the semi-quantity of Colloidal gold of the electron microscopy between the two groups was statistically significant as well. CONCLUSION: The above data suggest that AQP1 positive reaction is one of the sensitive signs of drowning and it is located in the type I alveolar cells.