| 鼻咽癌抗独特型单克隆抗体的制备和鉴定 |
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| 引用本文： | 检剑飞 王飒. 鼻咽癌抗独特型单克隆抗体的制备和鉴定[J]. 标记免疫分析与临床, 1994, 1(2): 79-83 |
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| 作者姓名： | 检剑飞 王飒 |
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| 作者单位： | 湖南医科大学肿瘤研究所 |
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| 摘 要： | 用针对鼻咽癌癌细胞相关抗原的单克隆抗体Ｆｃ１（Ａｂ１）作免疫原，采用常规免疫和杂交瘤制备方法，获得了一株抗Ｆｃ１Ｖ区独特型的杂交瘤：２Ａ９。双夹心ＥＬＩＳＡ显示２Ａ９所分泌的抗体（Ａｂ２）对Ｆｃ１有效强的亲和力。ＥＬＩＳＡ结合抑制试验结果显示，Ａｂ２能抑制Ｆｃ１对鼻咽癌细胞ＣＮＥ１的反应。这就证明Ａｂ２作用于Ｆｃ１抗体的Ｖ区。用Ａｂ２与钥孔戚血蓝素（ＫＬＨ）交联物免疫小鼠产生的抗－抗独特型抗体（Ａｂ３）的血清，能与Ｆｃ１竞争ＣＮＥ１细胞株上的原始靶抗原。免疫组化证实，Ａｂ３与Ｆｃ１对鼻咽癌细胞有相同的反应，均为细胞膜染色。可以看出Ａｂ３与Ａｂ１（Ｆｃ１）具有相同的配位。以上结果表明：２Ａ９杂交瘤细胞所分泌的抗独特型抗体Ａｂ２是带有鼻咽癌相关抗原内影像的单克隆抗体。
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| 关 键 词： | 鼻咽肿瘤 免疫疗法 单克隆抗体 制备 |
Preparation and characterization of Anti-idiotype Monoclonal Antibodies Bearing The Internal Image of Nasopharyngeal Cancer Associated Antigen |
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| Yan Jianfei,Wang Sa,Sun Qubing,et al.. Preparation and characterization of Anti-idiotype Monoclonal Antibodies Bearing The Internal Image of Nasopharyngeal Cancer Associated Antigen[J]. Labeled Immunoassays and Clinical Medicine, 1994, 1(2): 79-83 |
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| Authors: | Yan Jianfei Wang Sa Sun Qubing et al. |
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| Abstract: | A murine anti-idiotype monoclonal antibodies (Ab2) was produced by hybridoma 2A9 established through immunization with a murine IgG1 monoclonal antibody (Fc1) recognizing human nasopharyngeal cancer associated antigen. The binding inhibition assay indicated that Ab2 could inhibite the reactivity of Fc1 with CNE1. Sera from Ab2 immunized mice which contained anti-anti-idiotype antibodies (Ab3),could compete with Fc1 for the antigen on CNE1 by competitive inhibition assay. Both Ab3 and Fc1 showed immunoreactivity with the cell membrane of nasopharyngeal cancer cells. Therefore,the McAbs secreted by hybridoma 2A9 are the anti-idiotype antibodies bearing the internal image of the epitope of nasophargngeal carcinoma associated antigen. |
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| Keywords: | Antibodies. Monoclonal Antibodies. anti-idiotypic Nasopharyngeal Neoplasms Antibodies.anti-idiotypic antibody |
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