谷胱甘肽对高糖时人内皮细胞分泌前列环素和内皮素-1的影响

了解谷胱甘肽（ＧＳＨ）对高糖时人内皮细胞分泌前列环素（ＰＧＩ２）和内皮素－１（ＥＴ－１）的影响。方法： 原代培养人脐静脉内皮细胞，分别加入正常对照组，高糖组，正常＋ＧＳＨ组，高糖＋ＧＳＨ组培养基，放免法测定培养 ２４ｈ，４８ｈ，７２ｈ时培养基中的ＰＧＩ２和ＥＴ－１含量。结果：①高糖组较正常对照组ＰＧＩ２显著下降（Ｐ＜０．０１），ＥＴ－１显 著升高（Ｐ＜０．０１）；②高糖＋ＧＳＨ组较高糖组ＰＧＩ２升高（Ｐ＜０．０１），ＥＴ－１显著下降（Ｐ＜０．０１）。结论：ＧＳＨ在调节高 糖时人血管内皮细胞分泌 ＰＧＩ２／ＥＴ－１的平衡方面起一定作用。

Influence of reduced glutathione on prostacyclin and endothelin-1 secre-tion of human vascular endothelial cells cultured with a high concentra-tion of glucose

Aim: To investigate the influence of reduced glutathione (GSH) on prostacyclin (PGI2) and endothelin-1 (ET-1)secretion of human umbihcal vein endothehal cells (HUVEC) cultured with a high concentration of glucose. Methods: The medi-mn PGI2 and ET-1 were assayed by RIA in HUVEC which were cultured at 24,48 and 72 hours. Results: ① the content of PGI2in the high glucose group was lower than that in normal contrast group ( P < 0.01), and the content of ET-1 was higher ( P <0.01); ②the content of PGI2 in the high glucose with reduced glutathione group was higher than that in the high glucose group (P < 0.05), and the content of EI-i was lower (P < 0.01). Conclusion: GSH can balance the secretion of PGI2/ET-1 by HU-VEC.