复方丹参滴丸对人血管内皮细胞功能及形态保护作用的研究

目的： 观察复方丹参滴丸对细菌内毒素（LPS）所致人血管内皮细胞功能及形态结构损伤的作用。 方法： 人脐静脉内皮细胞培养，建立脂多糖损伤模型（以10 mg/L的LPS培养液培养细胞12 h），按分组分别把不同浓度的丹参滴丸（1 g/L、0.5 g/L、0.25 g/L、0.1 g/L）于损伤前、损伤后加入，分别进行细胞活力、一氧化氮（NO）、一氧化氮合酶（NOS）、内皮素（ET-1）、细胞内钙浓度的测定及倒置显微镜、倒置相差显微镜、激光共聚焦显微镜、透射电镜等形态学指标观察。 结果： 损伤后给不同浓度丹参滴丸对LPS所致人血管内皮细胞损伤均有明显减轻（P<0.05）。其中0.5 g/L作用最为突出(P<0.01)。损伤前给药只有浓度0.5 g/L、0.25 g/L对LPS所致内皮细胞损伤有明显减轻(P<0.05)，而高浓度(1 g/L)、低浓度(0.1 g/L)虽亦有减轻，但无统计学意义(P>0.05)。损伤前、后分别给予不同浓度滴丸后，LPS所致血管内皮细胞形态结构损伤均有明显减轻。 结论： 丹参滴丸对血管内皮细胞具明显保护作用。

The protective effect of composite salviae dropping pills on human umbilical vein endothelial cells injured by lipopolysaccharide

AIM: To observe the function and morphological change of human umbilical vein endothelial cells(HUVECs) treated with lipopolysaccharide(LPS) and composite salviae dropping pills(DSP).METHODS: HUVECs were cultured and incubated within 10 mg/L LPS for 12 hours.Different final concentrations of composite salviae dropping pills(1 g/L,0.5 g/L,0.25 g/L,0.1 g/L) were added before and after LPS treatment. Cell viability,NO,NOS,ET-1 and intracellular calcium were measured.The cells were observed under inverted microscope,inverted phase contrast microscope,laser confocal scanning microscopy and transmission electron microscope.RESULTS: When given after LPS treatment,different final concentrations of composite salviae dropping pills played a protective role(P<0.05), and the concentration of 0.5 g/L was the most effective(P<0.01).When given before LPS treatment,0.5 g/L and 0.25 g/L of composite salviae dropping pills protected the human vascular endothelial cells(P<0.05),but 1 g/L and 0.1 g/L didn't play a protective role(P>0.05).The HUVECs injured by LPS underwent apparent morphological change after treatment with composite salviae dropping pills.CONCLUSION: Composite salviae dropping pills have an evident protective effect on human umbilical vein endothelial cells exposed to LPS.