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四季豆蛋白抗生育作用的研究
引用本文:孙承琦,王淑如,胡卓逸,邹振伟,陈琼华. 四季豆蛋白抗生育作用的研究[J]. 药学学报, 1983, 18(2): 81-85
作者姓名:孙承琦  王淑如  胡卓逸  邹振伟  陈琼华
作者单位:南京药学院生物化学教研室 南京(孙承琦,王淑如,胡卓逸,邹振伟),南京药学院生物化学教研室 南京(陈琼华)
摘    要:四季豆提取物对小鼠抗着床、抗早孕和中期引产均有明显效果,腹腔注射最低有效剂量为16.7 mg/kg体重(丙酮法)和33.3 mg/kg体重(盐析法)。四季豆提取物经羟基磷灰石柱层析分离和纯化,可得4个洗脱峰。其中0.1 M磷酸缓冲液洗脱峰经醋酸纤维素薄膜电泳,可得一条主要区带和一条痕迹区带;经聚丙烯酰胺凝胶电泳可得一条主要区带,另有二条痕迹区带。此主要组分给小鼠腹腔注射,其抗早孕最低有效剂量为1.67~3.33 mg/kg体重,比提取物效价提高10~20倍。实验证明四季豆抗生育成分是一种蛋白质。

关 键 词:四季豆  蛋白质  抗生育作用
收稿时间:1981-12-10

STUDIES ON ANTIFERTILITY ACTION OF PHASEOLUS V ULGARIS
SUN Cheng-qi,WANG Shu-ru,HU Zhuo-ye,ZOU Zhen-wei and CHEN Oiong-hua. STUDIES ON ANTIFERTILITY ACTION OF PHASEOLUS V ULGARIS[J]. Acta pharmaceutica Sinica, 1983, 18(2): 81-85
Authors:SUN Cheng-qi  WANG Shu-ru  HU Zhuo-ye  ZOU Zhen-wei  CHEN Oiong-hua
Abstract:The crude extract of Phaseolus vulgaris L. has been shown to have obvious effect on preventing implantation, terminating early pregnancy and mid-pregnancy in mice. The least effective dose was found to be 16.7 mg/kg body weight by intraperitoneal injection.The crude extract of Phaseolus vulgaris L. was further purified on hydroxyapatite column and four fractions were obtained. One of these fractions, peak Ⅲ, eluted by 0.1 M pH 6.8 phosphate buffer, showed a main band and a trace band on cellulose acetate electrophoresis. It showed a main band and two other trace bands by polyacrylamide electrophoresis. The least effective dose of the main component on terminanting early pregnancy in mice was 1.67-3.33 mg/kg body weight by intraperitoneal injection. Its potency is 10-20 times higher than the crude extract of Phaseoius vulgaris. Its chemical nature is a protein.
Keywords:Phaseolus vulgaris L.  Protein  Antifertility action  
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