用蛋白印迹试验检测精神分裂症患者血清中博尔纳病病毒-p24抗体的研究

目的：探讨博尔纳病病毒（BDV）感染在我国的流行情况及其与精神分裂症的相关性。方法：在对优化表达的GST－BDV－p24融合蛋白进行特异性鉴定的基础上，通过确定融合蛋白与第一抗体、第二抗体间的最佳反应条件，建立可行的检测BDV－p24特异抗体的蛋白印迹（Western-blot）方法，进而对黑龙江地区精神分裂症患者和正常人对照血清中BDV－p24特异性抗体进行了检测，并通过血清－GST蛋白吸收后W estern-blot实验对阳性血清进行了确认。结果：116例精神分裂症患者中检出BDV－p24阳性血清10例，阳性检出率为8.6%，而正常人血清标本中未检出阳性。结论：我国存在博尔纳病病毒的感染，博尔纳病病毒的感染可能与精神分裂症的发生有关。

Detection of Borna disease virus-p24 specific antibody in the sera of schizophrenic patients of China by means of Western-blot

Objective To investigate whether Borna disease virus (BDV) infection is related to the schizophrenic patients from China. Methods A reliable Western blot method for detection of BDV p24 antibody was established by adjusting the reaction conditions of BDV p24 recombinant protein and specific antibodies. The sera of schizophrenic patients and normal controls from Heilongjiang Province were screened for specific BDV p24 antibody by this method, and the BDV p24 antibody positive sera were confirmed by the Western blot method with sera GST protein absorption. Results Ten of 116 (8.6%) schizophrenic patients were found to be positive for BDV p24 specific antibody, while no BDV p24 specific antibody was found in sera of normal controls. Conclusion The results demonstrate that the Borna disease virus infection also exists in China, and the infection is possibly associated with schizophrenia in some way.