| PFGE分子分型法在副溶血弧菌腹泻散发病人中的应用研究 |
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| 引用本文： | 章红红,陈洪友,陈敏,詹铭,胡洪亮,苏靖华,傅慧琴.PFGE分子分型法在副溶血弧菌腹泻散发病人中的应用研究[J].中国卫生检验杂志,2011(5):1042-1044. |
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| 作者姓名： | 章红红 陈洪友 陈敏 詹铭 胡洪亮 苏靖华 傅慧琴 |
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| 作者单位： | 上海市浦东新区疾病预防控制中心;上海市疾病预防控制中心;上海交大医学院附属仁济医院; |
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| 基金项目： | 上海市浦东新区科技发展基金创新资金(医疗卫生类)项目(PKJ2010-Y17) |
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| 摘 要： | 目的:运用脉冲场凝胶电泳(PFGE)对腹泻门诊病人的副溶血弧菌分离株进行分子分型,对缺乏聚集性信息的病例开展实验室监测。方法:对2010年7月27日至29日分离到的40株副溶血弧菌进行PFGE分子分型,利用BioNu-merics分析软件对图谱进行聚类分析。结果:40株副溶血弧菌PFGE指纹图谱可分为16个PFGE型(P1-P16),其中有6个型别分别有多株菌株处于同一聚类(P1、P2、P3、P8、P10、P13),结果显示在不同的腹泻监测点该时段可能存在多起聚集性病例。结论:应用PFGE分子分型,通过实验室监测可提示可能出现聚集性病例或暴发,结合流行病学调查资料可分析和确诊聚集性病例事件发生。
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| 关 键 词： | 副溶血弧菌 脉冲场凝胶电泳(PFGE) 聚集性病例 同源性分析 |
PFGE molecular subtyping of Vibrio parahemolyticus in patients with diarrhea application |
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| ZHANG Hong-hong,CHEN Hong-you,CHEN Min,ZHAN Ming,HU Hong-liang,SU Jing-hua,FU Hui-qin.PFGE molecular subtyping of Vibrio parahemolyticus in patients with diarrhea application[J].Chinses Journal of Health Laboratory Technology,2011(5):1042-1044. |
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| Authors: | ZHANG Hong-hong CHEN Hong-you CHEN Min ZHAN Ming HU Hong-liang SU Jing-hua FU Hui-qin |
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| Institution: | ZHANG Hong-hong1,CHEN Hong-you2,CHEN Min2,ZHAN Ming1,HU Hong-liang3,SU Jing-hua1,FU Hui-qin1(1.Pudong District Center for Disease Control and Prevention of Shanghai,Shanghai 200136,China,2.Shanghai Center for Disease Control and Prevention,Shanghai 200336,3.Renji hospital,Shanghai Jiaotong university school of medicine,Shanghai 200001,China) |
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| Abstract: | Objective:To monitor the outbreaks which were the lack of aggregation by using pulsed-field gel electrophoresis(PFGE) molecular subtyping of Vibrio parahaemolyticus from sporadic cases with diarrhea.Methods: PFGE was applied to molecular subtype 40 Vibrio parahaemolyticus strains isolated from 27th July to 29th July and PFGE patterns were analyzed by BioNumerics software.Results: 16 PFGE patterns(P1-P16) existed in PFGE fingerprint map in which 6 patterns(P1,P2,P3,P8,P10 and P13) had more than two strains.T... |
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| Keywords: | Vibrio parahaemolyticus Pulse field gel electrophoresis(PFGE) Clustering cases Homology analysis |
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