| 多重荧光定量PCR同时检测霍乱弧菌、副溶血性弧菌和创伤弧菌的方法研究 |
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| 引用本文： | 李宏,杨大伟,刘云国,孙涛,王建广,雷质文,周裔斌,贾俊涛,姜英辉.多重荧光定量PCR同时检测霍乱弧菌、副溶血性弧菌和创伤弧菌的方法研究[J].中国卫生检验杂志,2011(5). |
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| 作者姓名： | 李宏 杨大伟 刘云国 孙涛 王建广 雷质文 周裔斌 贾俊涛 姜英辉 |
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| 作者单位： | 中国合格评定国家认可中心;山东出入境检验检疫局;安徽农业大学; |
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| 摘 要： | 目的:利用多重荧光定量PCR技术检测霍乱弧菌、副溶血性弧菌、创伤弧菌。方法:以溶藻弧菌等17种相关试验菌株做特异性检测;并将标准菌株稀释成不同梯度,做灵敏度检测。结果:与传统的检测方法相比,该方法有很好的特异性且灵敏度高,检测时间短并可节省人力,具有快速方便等优点。结论:适用于样品中霍乱弧菌、副溶血性弧菌和创伤弧菌的快速检测。检测限分别为:霍乱弧菌为280 cfu/ml;副溶血性弧菌为:100 cfu/ml;创伤弧菌为:450 cfu/ml。
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| 关 键 词： | 霍乱弧菌 副溶血性弧菌 创伤弧菌 多重荧光定量PCR TaqMan探针 |
Establishment of multiplex real-time PCR assay for detection of Vibrio cholerae,Vibrio parahaemolyticus and Vibrio vulnificus |
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| LI Hong,YANG Da-wei,LIU Yun-guo,SUN Tao,WANG Jian-guang,LEI Zhi-wen,ZHOU Yi-bin,JIA Jun-tao,JIANG Ying-hui.Establishment of multiplex real-time PCR assay for detection of Vibrio cholerae,Vibrio parahaemolyticus and Vibrio vulnificus[J].Chinses Journal of Health Laboratory Technology,2011(5). |
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| Authors: | LI Hong YANG Da-wei LIU Yun-guo SUN Tao WANG Jian-guang LEI Zhi-wen ZHOU Yi-bin JIA Jun-tao JIANG Ying-hui |
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| Institution: | LI Hong1,YANG Da-wei2,3,LIU Yun-guo2,SUN Tao2,WANG Jian-guang2,LEI Zhi-wen2,ZHOU Yi-bin2,JIA Jun-tao2,JIANG Ying-hui2(1.China National Accreditation Service for Conformity Assessment,Beijing 100062,China,2.Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,3.Anhui Agriculture University,Hefei 230000,China) |
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| Abstract: | Objective:Rapid detection of Vibrio cholerae;Vibrio parahaemolyticus and Vibrio vulnificus were established by multiplex real-time PCR assay.Methods: Seventeen test strains such as Vibrio alginolyticus was tested by specific detection.The different grades of standard strain were obtained by diluting and sensitivity was detected.Results: Compared with conventional method,the multiplex real-time PCR assay was specific and sensitively.Conclusion: The multiplex real-time PCR assay was rapid and accurate,with th... |
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| Keywords: | Vibrio cholerae Vibrio parahaemolyticus Vibrio vulnificus multiple real-time PCR TaqMan-based probe |
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