| 体外诱导兔胚胎干细胞分化为神经细胞 |
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| 引用本文： | 曾现伟,吕翠,胡振波,潘顺,季泰令,杨斌. 体外诱导兔胚胎干细胞分化为神经细胞[J]. 中国卫生检验杂志, 2011, 0(6) |
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| 作者姓名： | 曾现伟 吕翠 胡振波 潘顺 季泰令 杨斌 |
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| 作者单位： | 潍坊医学院附属医院; |
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| 摘 要： | 目的:探讨体外诱导兔胚胎干细胞(ESCs)分化为神经细胞的方法。方法:应用多种诱导剂联合诱导兔ESCs,免疫荧光和流式细胞仪检测巢蛋白(Nestin)的表达。结果:诱导后兔ESCs能分化为具有典型神经元形态的细胞,Nestin阳性表达。结论:在体外采用定向分化诱导,兔ESCs可直接定向分化为神经干细胞。
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| 关 键 词： | 胚胎干细胞 神经干细胞 分化 兔 |
Differentiation of rabbit embryonic stem cells into neural stem cells in vitro |
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| ZENG Xian-wei,LV Cui,HU Zhen-bo,PAN Shun,JI Tai-ling,YANG Bin. Differentiation of rabbit embryonic stem cells into neural stem cells in vitro[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(6) |
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| Authors: | ZENG Xian-wei LV Cui HU Zhen-bo PAN Shun JI Tai-ling YANG Bin |
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| Affiliation: | ZENG Xian-wei,LV Cui,HU Zhen-bo,PAN Shun,JI Tai-ling,YANG Bin(Affiliated Hospital of Weifang Medical College,Weifang 261031,China) |
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| Abstract: | Objective:To explore the way of inducing rabbit embryonic stem cells(ESCs)differentiation into neural stem cells in vitro.Methods: The rabbit ESCs were induced into neurons by using different inducers.Then the expression of Nestin was detected by immunofluorescence methods and flow cytometry.Results: Under the inverted microscope,the induced ESCs showed typical neuronal morphological characteristics after induction.The induced cells also expressed Nestin.Conclusion: The rabbit ESCs can differentiate into ne... |
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| Keywords: | Embryonic stem cells Neural stem cells Differentiation Rabbit |
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