| 溶藻弧菌TaqMan实时PCR快速检测体系的建立 |
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| 引用本文： | 王海波,张京云,王多春,阚飙. 溶藻弧菌TaqMan实时PCR快速检测体系的建立[J]. 中国卫生检验杂志, 2011, 0(5) |
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| 作者姓名： | 王海波 张京云 王多春 阚飙 |
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| 作者单位： | 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室; |
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| 基金项目： | 传染病防治重大专项课题(2008ZX10004-002); 国家自然科学基金课题(0872260) |
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| 摘 要： | 目的:建立溶藻弧菌TaqMan实时PCR快速检测体系。方法:根据溶藻弧菌胶原酶基因colH的保守序列设计一对引物和一条TaqMan探针,优化反应体系中的引物浓度和探针浓度,评价此反应体系的特异度,并确定其检测下限。结果:优化的反应体系中,引物浓度为200 nmol/L,探针浓度为200 nmol/L,检测下限为1.0×102拷贝/μl,较普通PCR提高了100倍。特异度为100%。结论:本研究所建立的检测体系能够灵敏和特异地检测溶藻弧菌,可以作为溶藻弧菌的快速检测方法。
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| 关 键 词： | 溶藻弧菌 TaqMan实时PCR 快速检测 |
Establishment of TaqMan real-time PCR assay for the rapid detection of Vibrio alginolyticus |
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| WANG Hai-bo,ZHANG Jing-yun,WANG Duo-chun,KAN Biao. Establishment of TaqMan real-time PCR assay for the rapid detection of Vibrio alginolyticus[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(5) |
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| Authors: | WANG Hai-bo ZHANG Jing-yun WANG Duo-chun KAN Biao |
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| Affiliation: | WANG Hai-bo,ZHANG Jing-yun,WANG Duo-chun,KAN Biao(State Key Laboratory for Infectious Disease Prevention and Control.National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention.155 Changbai Road,Changping District,Beijing 102206,China) |
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| Abstract: | Objective:To establish TaqMan real-time PCR assay for the rapid detection of Vibrio alginolyticus.Methods: A pair of primers and a TaqMan probe were designed based on the sequence of the conserved region of collagenase gene colH.Concentrations of the primers and probe were optimized,the specificity of the detection system were evaluated,and the limit of detection was validated.Results: In the optimized detection system,the concentrations of each primer were 200 nmol/L,and the probe 200 nmol/L.The low limit ... |
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| Keywords: | Vibrio alginolyticus TaqMan real-time PCR Rapid detection |
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