| 儿科铜绿假单胞菌的OprD基因突变及表达对碳青霉烯类抗生素耐药的作用 |
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| 引用本文： | 李方去,刘洋,杨锦红,蒋伟燕,李向阳.儿科铜绿假单胞菌的OprD基因突变及表达对碳青霉烯类抗生素耐药的作用[J].中国卫生检验杂志,2011(5). |
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| 作者姓名： | 李方去 刘洋 杨锦红 蒋伟燕 李向阳 |
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| 作者单位： | 温州医学院附属第二医院检验科; |
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| 摘 要： | 目的:探讨我院儿科病区分离的碳青霉烯类耐药铜绿假单胞菌(CRPA)流行情况及OprD基因突变及表达在碳青霉烯类耐药中的作用。方法:儿科分离的30株碳青霉烯类耐药的铜绿假单胞菌,随机扩增DNA多态性(RAPD)进行同源性分析;PCR法检测KPC、IMP、VIM、SPM等产酶基因和OprD基因并双向测序及比对;FQ-RT-PCR测定OprD基因的mRNA相对表达量;SDS-PAGE电泳分析OprD外膜蛋白缺失情况。结果:儿科病区30株CRPA中,检出IMP基因阳性菌株1株,接合试验阴性。oprD基因缺失4株(4/30),突变率100%(9/9),碱基突变主要集中在1010 bp~1136 bp和1212 bp~1355 bp区域。CRPA菌株的oprD基因相对表达量与非耐药组差异有显著性(t=6.145,P<0.05),oprD基因突变位点与其表达量之间无相关性。结论:oprD基因发生广泛突变及表达量降低或缺失是本院儿科CRPA菌株对碳青霉烯类抗生素耐药的主要原因之一。
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| 关 键 词： | 假单胞菌 铜绿 碳青霉烯类 OprD蛋白 儿科 |
The role of mutation of OprD gene and expression to the resistant mechanisms of carbapenem in pseudomonas aeruginosa Isolates from pediatrics |
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| LI Fang-qu,LIU Yang,YANG Jin-hong,JIANG Wei-yan,LI Xiang-yang.The role of mutation of OprD gene and expression to the resistant mechanisms of carbapenem in pseudomonas aeruginosa Isolates from pediatrics[J].Chinses Journal of Health Laboratory Technology,2011(5). |
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| Authors: | LI Fang-qu LIU Yang YANG Jin-hong JIANG Wei-yan LI Xiang-yang |
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| Institution: | LI Fang-qu,LIU Yang,YANG Jin-hong,JIANG Wei-yan,LI Xiang-yang(Department of Clinical Laboratory,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China) |
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| Abstract: | Objective:To explore the antibiotic resistance mechanism and molecular epidemiology of Carbapenem Resistance in Pseudomonas aeruginosa(CRPA) isolated from Pediatrics.Methods: The PCR was applied to detect the KPC,IMP,VIM,SPM and OprD genes from 30 CRPA isolated from Pediatrics.DNA sequence was proceeded to analysis nucleotide sequence of the oprD gene and the postive of the other genes and the deduced amino acid sequence.FQ-RT-PCR was used to examine OprD mRNA relative expression.We analysis the amount of o... |
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| Keywords: | Pseudomonas aeruginosa Carbapenem OprD protein Pediatrics |
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