| 内蒙古2009年甲型H1N1流感病原与血清学监测结果分析 |
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| 引用本文： | 李昕,董杰,雷霞,海岩,南晓伟,跃华. 内蒙古2009年甲型H1N1流感病原与血清学监测结果分析[J]. 中国卫生检验杂志, 2011, 0(3) |
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| 作者姓名： | 李昕 董杰 雷霞 海岩 南晓伟 跃华 |
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| 作者单位： | 内蒙古自治区疾病预防控制中心; |
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| 摘 要： | 目的:分析2009年内蒙古甲型H1N1流感流行特点和健康人群感染状况,为进一步应对流感大流行提供科学依据。方法:采用RT-PCR、Real-time PCR法进行核酸检测,阳性样本进行鸡胚病毒分离培养。人群血清采用血凝(HA)和血凝抑制(HI)试验进行人群抗体检测。结果:全区各网络实验室共检测标本7663份,核酸阳性1543份,阳性率为20.14%。其中季节性H1N1阳性68份,H3N2阳性158份,甲型H1N1阳性957份,B型阳性164份,甲型流感阳性但无法分亚型的192份,混合4份;对本实验室检出的阳性标本509份进一步进行病毒分离培养,结果获得阳性毒株162株,阳性分离率为31.83%。采用血凝(HA)和血凝抑制试验(HI)对分离毒株型别鉴定,结果甲型H1N1份155份,占阳性毒株的96.91%;HI方法检测人群标本472份,抗体阳性95份,阳性率20.13%。结论:内蒙古地区2009年7月以后流行的流感优势株为甲型H1N1流感病毒,高峰出现在10月、11月;人群血清抗体检测表明人群抗体阳性率普遍偏低,学生的抗体水平相对较高。
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| 关 键 词： | 甲型H1N1流感 病原 抗体 监测 |
Surveillance on pathogen and serology of the pandemic(H1N1) 2009 in Inner Mongolia in 2009 |
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| LI Xin,DONG Jie,LEI Xia,HAI Yan,NAN Xiao-wei,YUE Hua. Surveillance on pathogen and serology of the pandemic(H1N1) 2009 in Inner Mongolia in 2009[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(3) |
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| Authors: | LI Xin DONG Jie LEI Xia HAI Yan NAN Xiao-wei YUE Hua |
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| Affiliation: | LI Xin,DONG Jie,LEI Xia,HAI Yan,NAN Xiao-wei,YUE Hua(Inner Mongolia Center for Disease Control and Prevention,Huhhot 010031,China) |
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| Abstract: | Objective:To analyze epidemical characteristic of the pandemic(H1N1) 2009 and to study infection situation on health people in Inner Mongolia,in 2009.This study can provide scientific basis for defending influenza pandemic effectively.Methods:Real-time RT-PCR and RT-PCR were used to detect nucleic acid of influenza virus from specimens.The viruses were inoculated and isolated with embryonated egg from the positive specimens.And,the hamagglutination(HA) and the hamagglutination inhibition(HI) tests were used... |
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| Keywords: | The Pandemic(H1N1) 2009 Pathogen Antibody Surveillance |
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