Matrix metalloproteinases-I, -3 and -8 and myeloperoxidase in saliva of patients with human immunodeficiency virus infection |
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| Authors: | L Mellanen,T Ingman,J Lä hdevirta,A Lauhio,A Ainamo,YT Konttinen,A Sukura,T Salo,T Sorsa |
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| Affiliation: | Departments of Periodontology and Medical Chemistry, University of Helsinki;Aurora Hospital, Helsinki;Department of Bacteriology and Immunology, University of Helsinki, Helsinki;Department of Anatomy, University of Helsinki, Helsinki;IV Department of Medicine, Helsinki University Central Hospital, Helsinki;Department of Veterinary Medicine, University of Helsinki, Helsinki;Department of Pedodontics and Orthodontics, University of Helsinki, Helsinki;Departments of Oral Surgery and Pathology, University of Oulu. Oulu, Finland |
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| Abstract: | OBJECTIVE: Human immunodeficiency virus (HIV)-sero-positive patients have frequently severe gingival inflammation andlor attachment loss. In addition many infectious diseases affect their periodontium with varying clinical manifestations. Matrix metalloproteinases seem to play a key role in physiological periodontal remodelling and pathological tissue destruction, The aim of the present study was to characterize the presence, molecular forms, cellular sources, activities, and relative amounts of fibroblast-type (matrix metalloproteinase [MMPJ-I) and neutrophil (MMP-8) collagenases, as well as their potential activator stromelysin-I (MMP-3) and myeloperoxidase in saliva of HIV-seropositive patients at different phases of HIV-infection. HIV-seronegative, healthy, age-matched patients served as controls.
PATIENTS AND METHODS: Saliva samples were characterized by Western blotting using antibodies specific for MMP-I, MMP-3 and MMP-8. Interstitial collagenase activities were measured using quantitative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis/laser densitometry assay. Myeloperoxidase was analysed using quantitative dot blotting. RESULTS: Clinical and microbiological evaluation of HIV-seropositive patients' periodontium showed the presence of putative periodontopathogens ie Actinobacillus actinomycetemcomitans (Ao), Porphyromonos gingivalis (Pg), Prevotella intermedia (Pi), Peptostreptococcus micros (Psm) and Campylobacter rectus (Cr) in their periodontal pockets. The amount of Candida increased with the severity of HIV-infection. Clinical and microbiological findings of HIV-seropositive patients suggested that they have a tendency to develop periodontal disease. Interstitial collagenase activities were found to be increased in saliva of different phases of HIV-infected patients compared to the controls. Independent of the phase of HIV- |
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