红景天苷抗大鼠肝星状细胞氧应激脂质过氧化作用的研究

目的:探讨红景天苷(Salidroside,SDS)对大鼠肝星状细胞(hepatic stellate cells,HSC)氧应激所致脂质过氧化的作用.方法:HSC与FeNTA共同培养产生氧应激.以MTT法测定红景天苷对HSC增殖的效应;LDH法测定红景天苷对HSC的毒性作用;以ELISA法测定细胞培养液中Ⅰ型胶原的水平;以试剂盒分别测定细胞培养液中MDA、GSH、GSH-PX、SOD水平.结果:HSC与FeNTA共同培养后,细胞内MDA、GSH水平明显升高,GSH-PX、SOD活力明显降低,并能明显促进HSC增殖和提高细胞培养上清中Ⅰ型胶原的水平.红景天苷可以逆转上述所有FeNTA所致效应.结论:红景天苷抗肝纤维化作用可能与其抑制脂质过氧化有关.

Study on Effects of Salidroside on Lipid Peroxidation on Oxidative Stress in Rat Hepatic Stellate Cells

OBJECTIVE: To investigate the effect of antioxidant Salidroside on the lipid peroxidation of oxidative stress in rat hepatic stellate cells in vitro. METHODS: HSC-T6 were incubated with 0.1 mM ferric nitrilotriacetate complex (FeNTA). MTT colorimetry was used for assaying proliferation of HSC. Cytotoxicity was measured by LDH colorimetry. Collagen type I accummulation in the culture media was measured by ELISA. Intracellular MDA, SOD, GSH and GSH-PX levels in the culture media were measured by their reagent boxes. RESULTS: HSC incubation with FeNTA resulted in a significant production of intracellular MDA and GSH, associated with decreasing SOD and GSH-PX activity. Exposure of HSC to FeNTA significantly enhanced the number of proliferating HSC and collagen type I levels in the culture medium. All these effects were reversed by the antioxidant Salidroside. CONCLUSION: The mechanism of Salidroside on fibrosis may be related to inhibit lipid peroxidation.