瑞芬太尼预处理对急性失血性休克家兔肺组织MDA、SOD、NO的影响

目的观察瑞芬太尼预处理对急性失血性休克家兔肺组织MDA、SOD、NO的影响。方法 40只家兔随机均分为三组：低剂量瑞芬太尼组（R1组,n=16）放血前15min持续泵入瑞芬太尼0.66μg/kg/min,分为两个亚组：休克1h组（RO1组,n=8）,休克2h组（RT1组,n=8）;高剂量瑞芬太尼组（R2组,n=16）放血前15min持续泵入瑞芬太尼1.32μg/（kg·min）：分为两个亚组,休克1h组（RO2组,n=8）,休克两小时组（RT2组,n=8）。生理盐水对照组（C组,n=8）给予等容量生理盐水,按照W igger＇s改良法制作家兔急性失血性休克模型。实验结束时取家兔右肺上叶测量湿干重比（W/D）,并检测家兔肺组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）的浓度。结果与C组比较,R1组和R2组的肺干湿重比降低;R1组和R2组的MDA含量降低、SOD活性升高、NO活性升高（P〈0.05）。与RO2组比较,RO1组、RT1组、RT2组MDA含量升高、SOD活性降低（P〈0.05）结论瑞芬太尼预处理,可使失血性休克家兔肺组织中MDA含量降低,SOD和NO的浓度升高。

Effects of remifentanil pretreatment on MDA, SOD and NO in rabbits' lung tissue during acute hemorrhagic shock

Objective To observe the effects of remifentanil pretreatment on MDA,SOD and NO in rabbits＇ lung tissue during acute hemorrhagic shock.Methods 40 healthy rabbits were randomly divided into three groups： Low-dose remifentanil group（R1 group,n= 16） that remifentanil 0.66μg/kg/min was pumped 15 minutes before bleeding.According to shock time R1 group was divided into one-hour group（RO1,n=8） and two-hour group（RT1,n=8）;High-dose remifentanil group（R2 group,n=16） R2 group remifentanil 1.32μg/kg/min was pumped 15 minutes before bleeding.According to shock time R2 group was divided into one-hour group（RO2,n=8） and two-hour group（RT2,n=8）;Control group NS（group C,n=8）.NS continued to pump into same volume of normal saline.The model of acute hemorrhagic shock was established by modified Wigger＇s methods.After experiment,lung tissue wet-weight /dry-weight ratio（W/D）,myeloperoxidase（MPO） activity,malondialdehyde（MDA） and the changes of circulating nitric oxide（NO） was measured.Results In group R1 and R2 the W/D lower than group C;in group R1 and R2 the activity of SOD and NO was higher than before,while MDA was abated（P〈0.05）.To compare with RO2 group,content of MDA were significantly higher,and the activity of SOD decreased in group RO1,RT1 and RT2.Conclusion Remifentanil pretreatment may decrease MDA content,increase SOD and NO activity in rabbits lung tissue during the accurate hemorrhagic shock.