HPLC色谱法同时测定川产虎杖中白藜芦醇、白藜芦醇苷及大黄素苷的含量

目的:采用HPLC色谱法,建立同时测定虎杖中白藜芦醇、白藜芦醇苷及大黄素苷(大黄素-8-O-β-D-葡萄糖苷)含量测定方法。方法:选用十八烷基硅烷键合硅胶为填充剂(C18柱250×4.6,5μm),以甲-醇0.8%磷酸水溶液梯度洗脱,0~50 min,甲醇43%~100%,流速为1.0ml/min,检测波长为284nm。结果:白藜芦醇在0.30μg~2.50μg范围内呈良好的线性关系,平均回收率为101.68%(n=5);白藜芦醇苷在0.37μg~3.10μg范围内呈良好的线性关系,平均回收率为100.05%(n=5);大黄素苷在0.24μg~2.00μg之间呈良好的线性关系,平均回收率为99.82%(n=5)。结论:该方法操作简便,结果准确可靠,适于中药虎杖中白藜芦醇、白藜芦醇苷及大黄素苷的同时测定,为含虎杖的药品质量控制提供一定的参考价值。

Simultaneous Determination of Resveratrol and Polydatin in the Roots of Polgonum Cuspidatum by HPLC

Objective： To set up the simultaneous determination method for determining the resveratorl and Polydatin in the roots of Polygonum cuspidatum. Methods： The HPLC system consists of a reverse-phase C18 Hyperrsil column（250）〈4. 6 5 μl） and a mobile phase of methanol-water（0. 8%phosphoric acid） gradient elution, and velocity of flow as 1.0 ml / min, with UV detective wavelength being 284nm. Results： Through investigation, this study shows that the resveratrol was linear within the range of 0. 30-2. 50 μg, the recovery was 101.68% （n = 5） ; the polydatin was linear within the range of 0. 37-3. 10 μg and the recovery was 100. 05%. The emodin-8-O-β-D-glucopyranosid was linear within the range of 0. 24-2.00 μg and the recovery was 99.82%. Conclusion： The method is simple and accurate, and suitable for Polygonum from veratryl alcohol and resveratrol as well, providing a reference value for the quality control of drugs containing Polygonum.