燃煤型砷中毒皮肤病变中P16,P21^WAF1／CIP1,CyclinD1蛋白 …

目的 探讨砷中毒与皮肤病变的关系。方法 用免疫组织化学法检测燃煤型砷中毒患者皮肤病变中Ｐ１６、Ｐ２１＾ＷＡＦ１／ＣＩＰ１、ＣｙｃｌｉｎＤ１蛋白的表达情况。结果 Ｐ１６的表达强度和密度随患者皮肤病变程度的加重而逐渐减弱,Ｐ１６蛋白在癌变组（Ａ组）、癌前病变组（Ｂ组）和过度角化组（Ｃ组）中普遍缺失表达,明显低于一般增生病变组（Ｄ组）和正常组;Ｐ２１＾ＷＡＦ／ＣＩＰ１、ＣｙｃｌｉｎＤ１的表达强度和密度随

The expression of P16, P21(WAF1/CIP1) and Cyclin D1 proteins in skin of patients with arseniasis caused by coal-burning and their significance

OBJECTIVE: To study the relationships between arseniasis and skin carcinoma. METHODS: The expressions of p16, p21(WAF1/CIP1) and Cyclin D1 proteins in skin of arsenic intoxication patients caused by coal-burning was measured by immunohistochemistry methods. RESULTS: The intensity and density, and the positive percentage of p16 protein were gradually reduced as the skin pathological changes progressed. Expressions of p16 protein was lower in the carcinoma group (A group), pre-carcinoma group (B group) and overkeratosis group (C group) than the general cell proliferation group (D group) and normal group (P < 0.05, P < 0.01). On the other hand, the cell density and the positive percentage of p21(WAF1/CIP1) and Cyclin D1 proteins were gradually increased as the skin pathological changes progressed. The expression of p21(WAF1/CIP1) protein in all pathological groups were higher than the normal group (P < 0.05, P < 0.01), and expression of CyclinD1 protein in A, B and C groups were higher than the D group and normal group (P < 0.05, P < 0.01). CONCLUSIONS: Skin overkeratosis may be the earlier stage of skin carcinoma, lower expression of p16 protein and overexpression of Cyclin D1 protein may be considered as prognostic biomarkers to skin carcinogesis; p16, p21(WAF1/CIP1) and Cyclin D1 may play important role in the cooperative way in the development of arseniasis caused by coal-burning and further progression to skin carcinoma. Monitoring of the expression of p16, p21(WAF1/CIP1) and Cyclin D1 proteins may be valuable for early detection and early treatment and prognostic assessment of skin carcinoma caused by coal-burning.