| L苯丙氨酸抑制自发性高血压大鼠心肌成纤维细胞增殖 |
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| 引用本文： | 李振波 赵光胜. L苯丙氨酸抑制自发性高血压大鼠心肌成纤维细胞增殖[J]. 高血压杂志, 1999, 7(2): 163-165 |
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| 作者姓名： | 李振波 赵光胜 |
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| 作者单位： | 上海第二医科大学附属瑞金医院上海市高血压研究所(李振波)，中国医学科学院北京天坛医院(赵光胜) |
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| 摘 要： | 目的旨在观察Ｌ－苯丙氨酸对自发性高血压大鼠心肌成纤维细胞的增殖作用的影响。方法取１天龄自发性高血压大鼠心脏组织培养心肌成纤维细胞，分别以不同浓度的Ｌ－苯丙氨酸、酪氨酸和组氨酸干预一定时间后，利用３Ｈ－胸腺嘧啶掺入技术和细胞计数测定各不同浓度的氨基酸对心肌成纤维细胞ＤＮＡ合成和细胞增殖数目的影响。结果随着Ｌ－苯丙氨酸浓度的增加，心肌成纤维细胞的ＤＮＡ合成量减少，细胞增殖数目减低。而Ｌ－酪氨酸和组氨酸随着浓度的增加对心肌成纤维细胞ＤＮＡ的合成量和细胞增殖数目无影响。结论Ｌ－苯丙氨酸可以特异性地抑制ＳＨＲ心肌成纤维细胞的增殖。
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| 关 键 词： | L－苯丙氨酸；自发性高血压大鼠；心肌成纤维细胞；DNA合成；细胞培养 |
LPhenylalanine Specifically Inhibited the Proliferation of Myocardial Fibroblast in Spontaneously Hypertensive Rat |
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| LI Zhenbo,ZHAO Guangsheng=. LPhenylalanine Specifically Inhibited the Proliferation of Myocardial Fibroblast in Spontaneously Hypertensive Rat[J]. Chinese Journal of Hypertension, 1999, 7(2): 163-165 |
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| Authors: | LI Zhenbo ZHAO Guangsheng= |
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| Abstract: | Objective To observe the effect of LPhenylalanine on proliferation of myocardial fibroblast in spontaneosly hypertensive rat. Methods Amino acids in different concerntration were added to myocardial fibroblast cultured from 1 dayold spontaneously hypertensive rat for a defined time. The3Hthymidine incorporation was applied to reflect the fibroblasts proliferation activity with counts of3Hthymidine per minute divided by the number of fibroblasts. Result The DNA synthesis/cell count were decreased with the Phenylalanine in a dose dependet manner, but not with tyrosine and histidine. Conclusion LPhenylalanine inhibited the proliferation of myocardial fibroblast in spontaneously hypertensive rat, which may explain the proven cases of phenylalanine preventing blood pressure rise and myocardial hypertrophy in spontaneously hypertensive rat. |
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| Keywords: | LPhenylalanine Spontaneously hypertensive rat myocardial fibroblast cell |
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