兔舌癌哨位淋巴结微转移模型的建立

目的:建立兔舌癌哨位淋巴结(sentinel lymph node,SLN)微转移模型,为进一步研究舌癌区域淋巴结转移的发生、发展机制及防治提供实验平台。方法:先正位、多循环移植Vx-2癌瘤于兔舌,每次8只兔,筛选出高淋巴转移潜力移植瘤;再将其移植到40只兔的舌部,分别于移植后第8、10、12、14和16天各随机处死8只,取出兔舌Vx-2移植瘤SLN,经连续切片和免疫组织化学检测其微转移形成情况。应用SPSS 17.0软件包对结果进行统计学分析。结果:经3次正位循环移植后,所得Vx-2移植瘤舌移植后SLN转移率达100%(8/8);该高淋巴转移潜力移植瘤移植兔舌后10~12 d,所获移植瘤SLN微转移发生率为100%(8/8)。结论:应用本研究筛选的高淋巴转移潜力Vx-2瘤移植兔舌后10~12 d,可获得较稳定的兔舌癌SLN微转移模型。

A rabbit model of micrometastasis in sentinel lymph node from tongue carcinoma

PURPOSE： To establish a rabbit model of micrometastasis in sentinel lymph node （SLN） from tongue carcinoma, so the mechanism of regional lymph node metastasis from tongue carcinoma as well as its management may be studied through this model. METHODS： First, lymph node metastatic specimen of rabbit tongue Vx-2 carcinoma was orthotopically and serially transplanted into the tongue of disease-free rabbit at 4-week interval （8 rabbits for each passage） for screening carcinoma specimens with high lymphatic metastasis potential. Then, the selected specimens were orthotopically re-transplanted into tongues of 40 disease-free rabbits; SLNs of the developed Vx-2 tumor were harvested at 8~, 10~, 12~, 14~ and 16~ day （8 rabbits for each harvest） respectively after the transplantation, and thorough work-up of the SLNs was done by serial sectioning and immunohistochemistry for evidence of micrometastasis. RxC table Chi- square test was used for comparison among multi-ratio using SPSS 17.0 software package. RESULTS： Increased SLN metastasis was noted with serial passages of transplanting lymphatic metastasis specimens in the tongue： 3/8 （37.5%） in the first passage, 5/8 （65%） in the second, 8/8 （100%） in both the third and the fourth. After orthotopic transplantation of the harvested nodal carcinoma specimen with high metastatic potential, micrometastasis was detected in SLN in 5/8 rabbits （62.5%） of group 8^th day, 8/8 （100%） of both group 10^th day and group 12^th day, 6/8 （75%） of group 14z day and 3/8 （37.5%） of group 16^th day respectively. CONCLUSION： Specimens with high lymphatic metastasis potential can be achieved by serial and orthotopic transplantation ot＂ lymphatic metastasis specimens from Vx-2 carcinoma in rabbit tongue; and the rabbit of group 10-12 days after orthotopic re-transplantation of them is an ideal model of micrometastasis in SLN from tongue careinoma.