NOD2在舌鳞癌中的表达特征及功能变化

目的:检测NOD2在舌鳞癌中的表达特征及功能变化。方法:采用免疫组织化学、RT-qPCR检测人舌鳞癌和正常舌黏膜组织中NOD2的表达;采用免疫荧光、RT-qPCR检测NOD2活化对人舌鳞癌细胞株Tca8113、正常舌黏膜上皮细胞分泌hBD-2的影响。应用SPSS 13.0软件包对各组mRNA的表达进行t检验及多个样本均数的方差分析。结果:①NOD2蛋白表达于细胞质,舌鳞癌组织中NOD2的蛋白表达率为64.0%(16/25),显著低于正常舌黏膜组织的表达率84.0%(21/25,P<0.05)。舌鳞癌组织NOD2 mRNA表达量为正常舌黏膜组织的(0.521±0.080)倍(P<0.05)。②hBD-2表达于细胞质,Tca8113细胞中hBD-2为低表达和阴性表达,MDP处理6 h、12 h后,Tca8113细胞中hBD-2mRNA的表达量较处理前显著上调(1.770±1.202)、(68.277±48.946)倍(P<0.05)。结论:舌鳞癌组织中NOD2 mRNA和蛋白表达显著低于正常舌黏膜组织,NOD2激动剂MDP能上调Tca8113细胞中hBD-2 mRNA和蛋白的表达。推测在舌黏膜中NOD2能调节hBD-2表达,可能影响舌鳞癌的免疫功能,与舌鳞癌进展相关。

Expression and functional changes of NOD2 in tongue squamous cell carcinoma

PURPOSE： This study aimed to investigate the expression and functional changes of NOD2 in tongue squamous cell carcinoma （TSCC）. METHODS： Immunohistochemical staining and RT-qPCR were employed to evaluate NOD2 expression in TSCC and non-cancer tongue epithelial tissues, hBD-2 production was investigated by immunofluorescenee staining and RT-qPCR. Student＇s t test was used to assess the statistical significance of mRNA expression between the two groups, and two-way analysis of variance was used to determine the differences among different groups using SPSS 13.0 software package. RESULTS： ①NOD2 was expressed in the cell plasma. NOD2 expression was significantly lower in TSCC tissues （64.0%, 16/25） than in non-cancer tongue epithelial tissues （84.0%, 21/ 25） （P〈0.05）. NOD2 messenger RNA expression in TSCC tissues was （0.521±0.080） times of that in non-cancer tongue epithelial tissues（P〈0.05）. ②hBD-2 was expressed in the cell plasma. When MDP was added to TcaS113 cells for 6,12 h, hBD-2 expression was significantly upregulated （1.770±1.202 and 68.277±48.946 times, P〈0.05）. CONCLUSION： NOD2 expression was significantly lower in TSCC, hBD-2 expression was markedly upregulated when NOD2 was activated by MDP. This suggests that NOD2 adjust hBD-2 expression in the tongue mucosa, and affect the immune function of TSCC, as well as the progression of TSCC.