小檗碱对早期ACM大鼠治疗效果及机制的研究

目的:观察小檗碱(BBR)对早期酒精性心肌病(ACM)大鼠的治疗作用,探讨其作用机制。方法:每日白酒灌胃以建立SD大鼠早期ACM模型,将已建模成功的28只SD大鼠随机分为小檗碱治疗组(14只)、安慰剂组(14只),并随机抽取10只正常SD大鼠作为正常对照组。干预4周后应用超声心动图测定两组大鼠心脏结构及功能参数,RT-PCR法检测心肌组织中MMP-2mRNA、TIMP-2 mRNA表达。结果:与对照组比较,安慰剂组LVEDD、LVESD、LA增大,左室舒张功能减低,心肌组织中的MMP-2 mRNA表达水平明显上升,TIMP-2mRNA表达水平下降,差异有统计学意义(P<0.05);与安慰剂比较,小檗碱组LVEDD、LVESD、LA减小,左室舒张功能有所改善,心肌组织中的MMP-2 mRNA表达水平显著下降,TIMP-2 mRNA表达显著升高(P<0.05)。结论:BBR逆转早期ACM大鼠心室重构,改善心功能,可能与减少心肌组织MMP-2表达有关。

The effect of the intervention of berberine in ACM rats

Objective To study the effects of berberine(BBR) in alcoholic cardiomyopathy rats and cardiomyopathy(ACM) model was established by gavage wine.The rats in ACM model(n = 28) inquire its functional mechanism.Methods The Sprague-Dawley(SD) rat of experimental alcoholic were divided into berberine treatment group(n = 14) and ACM control group(n = 14),and randomly selected 10 rats were as normal control group.After 8 weeks,echocardiography and TDI was used to evaluate the changes in cardiac structure and function.MMP-2gene and TIMP-2 gene expression was measured by real-time PCR.Results Compared with the control group,ACM control group LVEDD、LVESD、LA were increased,the left ventricular diastolic was decreased,MMP-2gene expression was significantly increased,TIMP-2gene expression reduced significantly.Compared with the ACM control group,berberine treatment group could improve obviously the function of heart of the rats,and MMP-2gene expression reduced significantly.Conclusion BBR can significantly improve the function of the ACM in rats,reverse its ventricular remodeling,was probably related with decreased the myocardial level of MMP-2.