RP-HPLC法测定复方金银花颗粒中黄芩苷的含量

目的：建立反相高效液相色谱法测定复方金银花颗粒中黄芩苷含量的方法。方法：采用Zorbax ODS（250mm×4.6mm,5μm）色谱柱,以甲醇：水：磷酸（47∶53∶0.2）为流动相,流速1.0 ml.min^-1,检测波长为280nm,用甲醇提取复方金银花颗粒中黄芩苷。结果：黄芩苷在12.65-63.25ng（r=0.9996）范围内线性关系良好,平均回收率为97.02%,RSD为1.37%。结论：本方法操作简便、精密度好,适用于复方金银花颗粒的质量控制。

Determination of the contents of scutellarin In Fufang Jinyinhua Keli by HPLC

OBJECTIVE To establish the HPLC method for determination of scutellarin in Fufang Jinyinhua Keli.METHODS：HPLC was performed on column of Zorbax ODS（250mm×4.6mm,5μm） with methanol-water-phosphoric acid（47∶53∶0.2） as mobile phase.Flow rate was 1.0 ml·min-1 and detecting wavelength was 280nm.Scutellarin in Fufang Jinyinhua Keli was extracted by methanol reflux method.RESULTS：The calibration curve for scutellarin was linear in the range of 12.65~63.25ng（r=0.9996）.The average recovery was 97.02% with RSD=1.37%.CONCLUSION：This method is simple,accurate,repeatable and effective for quality control of Fufang Jinyinhua Keli.