白血病抑制因子受体中gp130细胞内区诱导白血病细胞Meg-01内c-myc的表达

观察人白血病细胞系Ｍｅｇ－０１白血病抑制因子（ＬＩＦ）受体α亚基（ｇｐ１９０）和另一亚基ｇｐ１３０细胞内区与ｃ－ｍｙｃ的关系,以探明白血病细胞过度增殖和晚期分化异常的机制。方法：用基因重组技术将两基因细胞内区互换以构成两嵌合体受体（１９０／１３０,１３０１９０）,并分别在Ｍｅｇ－０１表达,其与

c-myc expression in leukemia Meg-01 induced by the homodimerization of the cytoplasmic region of gp130

Objective: To investigate the relation between the cytoplasmic region of each subunit of leukemia inhibitory factor(LIF) receptor and cell proliferation related gene, c myc expression level in human leukemia line Meg 01. Methods: We prepared the chimerical receptor exchanged cytoplasmic domain (190/130, 130/190) and separately expressed it on the membrane of Meg 01, then competitively bound LIF with the wild receptor. Therefore, the cytoplasmic region homodimerization (190cyt 190cyt, 130cyt 130cyt) initiated the intracellular signal transduction,the cell proliferation and differentiation. c myc expression level was observed by means of immunoblotting and immunohistochemistry in Meg 01. Results: Higher level of c myc and the faster cell proliferation were determined in group 190/130 compared with the wild type receptor subunit. Conclusion: The cytoplasmic domain of gp130 involves in the induction of c myc expression and the cell proliferation of Meg 01.