刺五加在心肌细胞缺血再灌注损伤中的保护作用

目的：研究刺五加注射液对缺血再灌注损伤(ischemia-reperfusion,I/R)心肌细胞的保护作用。方法：体外培养心肌细胞并计数；721可见光分光光度计测量培养液中的乳酸脱氢酶(LDH)、丙二醛(MDA)和超氧化物歧化酶(SOD)的含量；激光扫描共聚焦显微镜观察细胞内钙离子荧光染色。结果：中药刺五加注射液各剂量组与I/R组相比,细胞存活率升高,LDH、MDA水平降低,SOD水平升高,荧光像素值及阳性染色面积降低,有统计学意义。结论：中药刺五加注射液对缺血再灌注损伤心肌细胞有保护作用,可能与其保护细胞膜、清除氧自由基和减少钙离子内流等机制有关,其最佳浓度为40%。

Protecfive effect of acanthopanax on ischemia-reperfusion injury of myocardial cells

Objective:To study the mechanism of the protection of acanthopanax on ischemia-reperfusion injury of myocardial cells.Methods:Myocardial cells were cultured in vitro and numbered.LDH,MDA and SOD in the culture fluid were measured with 721 visible range spectrophotometer and the fluorescence staining of the intracel- lular calcium ion were observed with division reproduction laser confocal microscopy.Results:Compared to the I/R group,the 3 acanthopanax groups had a high survival rate of myocardial cell,and SOD value,and had a low LDH,MDA,fluorescence staining value and positive Ca~(2 ) staining areas.Conclusion:Acanthopanax has a pro- tective effect on I/R isolated myocardial cells,the best concentration of which is 40%.It may be its mechanism to protect the cell envelope,clean oxygen free radical and decrease Ca~(2 ) inflow.