中国藏族PSCA基因rs2294008多态性与胃癌遗传易感性的关系

目的:研究前列腺干细胞抗原基因(prostatestem cell antigen gene,PSCA)rs2294008位点多态性与中国藏族胃癌患者遗传易感性的关系.方法:收集185例藏族胃癌患者与200例健康人群的外周血样本,提取基因组DNA,采用dHPLC方法进行PSCA基因rs2294008位点分型.结果:PSCA基因rs2294008位点3种基因型C C、C T、T T在胃癌病例组中频率分别为:40.00%、48.65%和11.35%,而在对照组中分别为54.00%、39.50%和6.50%.与CC型比较,携带CT,TT型基因型者胃癌发生的危险性增加,OR值分别为1.66(95%CI 1.09-2.54)和2.36(95%CI 1.11-5.00).结论:PSCA基因rs2294008位点CT,TT基因型增加中国藏族人群的胃癌易感性.

Relationship between the rs2294008 polymorphism of the PSCA gene and susceptibility to gastric cancer in Tibetans

AIM: To investigate the relationship between the rs2294008 polymorphism of the PSCA gene and susceptibility to gastric cancer in Tibetans. METHODS: The rs2294008 genotypes were determined by PCR-based denaturing high-performance liquid chromatography and direct DNA sequencing in 185 sporadic cases of gastric cancer and 200 controls recruited in Tibet. RESULTS: The frequency of CC genotype was 40.00% in gastric cancer patients and 54.00% in normal controls,and the corresponding percentages for CT and TT genotypes were 48.65% and 39.50%,and 11.35% and 6.50%,respectively.Compared to CC genotype,CT and TT genotypes were associated with an increased risk of gastric cancer(OR = 1.66,95%CI 1.09-2.54;OR = 2.36,95%CI 1.11-5.00). CONCLUSION: The rs2294008 polymorphism of the PSCA gene is associated with increased susceptibility to gastric cancer in Tibetans.