儿童过敏性紫癜补体C4基因多态性分析

目的探讨补体C4基因多态性与中国汉族儿童过敏性紫癜(HSP)的关系。方法 HSP汉族儿童188例(HSP组)分为肾炎组(74例,合并紫癜性肾炎)和无肾炎组(114例,无肾炎)。健康汉族儿童130例为对照组。ELISA法检测HSP组血清补体C4水平。结果 HSP组C4AQ0频率高于对照组(7.98%vs.0.77%)(P<0.01)。肾炎组与无肾炎组的C4AQ0频率相仿(8.11%vs.7.89%)(P>0.05)。HSP组和对照组均无C4BQ0。HSP患儿中,C4AQ0患儿血清补体C4水平低于无C4AQ0者[(0.216±0.045)g/L vs.(0.262±0.064)g/L](P<0.05)。结论补体C4基因多态性与汉族儿童HSP的发生有关,提示C4AQ0可能是汉族儿童HSP的易感基因。

Analysis of genetic polymorphism of complement C4 in children with Henoch-Schonlein purpura

Objective To study the relationship of complement C4 genetic polymorphism with Henoch-Schonlein purpura(HSP) in children. Methods Complement C4 genetic polymorphism of 188 ethnic Han children with HSP(group HSP) was determined by PCR,of whom 74 children were with concurrent nephritis(group HSPN) and 114 children without nephritis(group NHSPN).Another 130 healthy children were taken as the controls(group C).Plasma complement C4 level was measured by ELISA. Results The frequencies of C4AQ0 in group HSP were significantly higher than those in group C(7.98% vs.0.77%)(P<0.01).The frequency of C4AQ0 in group HSPN was similar to that in group NHSPN(8.11% vs.7.89%)(P>0.05).There was no C4BQ0 in groups of HSP and C. Of HSP children,serum complement C4 level was lower in HSP children with C4AQ0 than that in those without C4AQ0[(0.216±0.045) g/L) vs.(0.262±0.064) g/L](P<0.05). ConclusionComplement C4 genetic polymorphism is associated with the incidence of HSP in Han children.C4AQ0 may be a susceptible gene of HSP in Han children.