| 2014年湖南省C亚属人腺病毒1株临床分离株的全基因组特征分析 |
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| 引用本文： | 马若群,向星宇,张红,李芳彩,黄超洋,崔爱利,张燕,许文波,毛乃颖,朱贞. 2014年湖南省C亚属人腺病毒1株临床分离株的全基因组特征分析[J]. 中华实验和临床病毒学杂志, 2021, 0(1): 49-56 |
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| 作者姓名： | 马若群 向星宇 张红 李芳彩 黄超洋 崔爱利 张燕 许文波 毛乃颖 朱贞 |
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| 作者单位： | 中国疾病预防控制中心病毒病预防控制所;湖南省疾病预防控制中心 |
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| 基金项目： | 国家重点研发计划(2018YFC1200602,2017YFC1200303);"十三五"国家科技重大专项(2017ZX10104001-002,2018ZX10713002,2018ZX10713001-003)。 |
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| 摘 要： | 目的:了解2014年湖南省C亚属人腺病毒(HAdV-C)分离株的基因特征。方法:2014年从湖南省长沙市严重急性呼吸道感染儿童病例咽拭子标本中分离到一株HAdV-C病毒株(Hunan2014-s024)，分段扩增目的基因片段，拼接后获得全基因组序列，同时下载GenBank数据库中国内外流行的HAdV-C代表株全基因组序...
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| 关 键 词： | 湖南省 C亚属人腺病毒 基因特征 |
Analysis of whole genome characteristics of 1 clinical subgenus C human adenovirus strain isolated from Hunan province in 2014 |
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| Ma Ruoqun,Xiang Xingyu,Zhang Hong,Li Fangcai,Huang chaoyang,Cui Ailii,Zhang Yan,Xu Wenbo,Mao Naiying,Zhu Zhen. Analysis of whole genome characteristics of 1 clinical subgenus C human adenovirus strain isolated from Hunan province in 2014[J]. Chinese journal of experimental and clinical virology, 2021, 0(1): 49-56 |
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| Authors: | Ma Ruoqun Xiang Xingyu Zhang Hong Li Fangcai Huang chaoyang Cui Ailii Zhang Yan Xu Wenbo Mao Naiying Zhu Zhen |
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| Affiliation: | (NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Hunan Center for Disease Control and Prevention,Changsha 410005,China) |
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| Abstract: | Objective To understand the genetic characteristics of subgenus C human adenovirus(HAdV-C)strain isolated in Hunan in 2014.Methods An HAdV-C strain named Hunan2014-s024 was isolated from throat swab collected from a child with severe acute respiratory infection in Changsha city of Hunan province in 2014.The whole genome sequence(WGS)was obtained by segmented amplification and sequence splicing.Database of HAdV-C was constructed with the strain from this study and the HAdV-C representative strains from GenBank.The whole genome characteristics was analyzed by using bioinformatics software.Results Strain Hunan2014-s024 has the highest homology with the HAdV-C strain(MK041244-CHN-2006)isolated from stool sample of a healthy child in Shanxi province in 2006,and the genome of strain MK041244-CHN-2006 was used as the backbone,and penton based gene was replaced by gene fragment from MK041246-chn-2012 strain isolated from a child with acute lower respiratory tract infection in Hunan province in 2012.Conclusions Our result indicated that 2014 Hunan strain(Hunan2014-s024)is a recombinant virus of 2006 Shanxi strain(MK041244-CHN-2006)and 2012 Hunan strain(MK041246-CHN-2012).Due to the change of pathogenicity,it is necessary to strengthen the molecular epidemiological surveillance of HAdV-C to understand its potential disease burden and public health significance. |
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| Keywords: | Hunan province Human mastadenovirus C Genetic characterization |
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