Absorption of protein via the intestinal wall. A quantitative model |
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| Authors: | E Ziv O Lior M Kidron |
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| Affiliation: | 1. Department of Food Science, Central Taiwan University of Science and Technology, Taichung City 406, Taiwan, ROC;2. Institute of Biochemistry and Biotechnology, Taiwan, ROC;3. Department of Physiology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City 40201, Taiwan, ROC;1. Molecular Mechanisms of Mycobacterial Infection, Center for Molecular Inflammation Research, Norwegian University of Science and Technology, Trondheim, Norway;4. Department of Developmental Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA;5. Laboratory for GPCR Biology, Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA;1. Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX;2. Department of Surgery, University of Texas Medical Branch, Galveston, TX;3. Shriners Hospitals for Children, Galveston, TX;4. Sulpizio Cardiovascular Center, University of California, San Diego, CA;5. University of Arkansas for Medical Sciences, Little Rock, AR |
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| Abstract: | Intact, biological active insulin and pancreatic RNase can be absorbed from the intestinal lumen into the blood circulation. The absorption is dependent on the addition of bile acid (sodium cholate) and proteinase inhibitor. The quantitative absorption of insulin and pancreatic RNase has been demonstrated in an in situ model. The amount of insulin absorbed after 30 min from the ileum to the mesenteric vein was 0.025% of the initial amount. Sodium cholate (10 mg/ml) and 3000 KIU/ml aprotinin enhanced this absorption by 30 times. The amount of pancreatic RNase which was absorbed from the ileum to the blood was 0.002% of the initial amount during 30 min. Sodium cholate (10 mg/ml) and 3000 KIU/ml aprotinin increased the absorption by a factor of 200. No damage occurred to the intestine during the experimental procedures. The sieving characteristics of the intestinal wall were not altered by the presence of sodium cholate and proteinase inhibitor in the intestinal lumen. These results suggest that sodium cholate and proteinase inhibitors can facilitate the absorption of intact, biologically active proteins across the intestinal wall. |
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