| 大鼠骨骼肌细胞不存在功能性β3—肾上腺素受体 |
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| 引用本文： | 高本波,韩启德.大鼠骨骼肌细胞不存在功能性β3—肾上腺素受体[J].中国药理学报,1998,19(6):531-534. |
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| 作者姓名： | 高本波 韩启德 |
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| 摘 要： |
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| 关 键 词： | β－肾上腺素 受体 骨骼肌 |
No functional beta 3-adrenergic receptors expressed in rat skeletal muscle cells. |
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| B B Gao,Q D Han.No functional beta 3-adrenergic receptors expressed in rat skeletal muscle cells.[J].Acta Pharmacologica Sinica,1998,19(6):531-534. |
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| Authors: | B B Gao Q D Han |
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| Institution: | Institute of Vascular Medicine, Third Hospital, Beijing Medical University, China. |
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| Abstract: | AIM: To determine the functional role of beta 3-adrenoceptors (beta 3-AR) in rat skeletal muscle cells. METHODS: Nonselective beta-AR agonist isoprenaline (isoproterenol, Iso), beta 3-AR agonist CGP12177A which is a beta 1-/beta 2-AR antagonist and selective beta 3-AR antagonist SR59230A on cAMP accumulation was studied in primary cultured rat skeletal muscle cells. RESULTS: Iso stimulated cAMP accumulation in a concentration-dependent manner with EC50 of 1.51 nmol.L-1 and propranolol inhibited cAMP accumulation stimulated by Iso with KB of 3.47 nmol.L-1. CGP12177A had no effect on cAMP accumulation but inhibited cAMP production induced by Iso. SR59230A 10 nmol.L-1 did not inhibit cAMP production induced by Iso. CONCLUSION: The functional beta 3-AR are not present or at least not coupled to adenylyl cyclase activity in skeletal muscle cells. |
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