| 左旋千金藤立定增强大鼠损毁侧纹状体DARPP—32蛋白磷酸化的作用 |
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| 引用本文： | 郭翔,金坚.左旋千金藤立定增强大鼠损毁侧纹状体DARPP—32蛋白磷酸化的作用[J].中国药理学报,1998,19(2):100-103. |
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| 作者姓名： | 郭翔 金坚 |
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| 作者单位： | [1]中国科学院上海药物研究所 [2]核医学国家重点实验研究室 |
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| 摘 要： | 研究左旋千金藤立定（ＳＰＤ）对羟多巴胺损毁大鼠纹状体中ＤＡＲＰＰ－３２蛋白磷酸化程度的影响。方法：反磷酸化测定脱磷ＤＡＲＰＰ－３２的含量，结果：ＳＰＤ不改变正常大鼠纹状体中ＤＡＲＰＰ－３２磷酸化的程度，但能拮抗Ｄ１激动剂的作用；对羟多巴胺损毁大鼠的损侧纹状体，ＳＰＤ使脱磷ＤＡＲＰＰ－３２的含量降低４４％，给予Ｄ１拮抗剂可以拮抗这一作用。结论：在损侧纹状体，ＳＰＤ显示Ｄ１激动剂的作用特性，增加ＤＡＰ
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| 关 键 词： | 千金藤立定 磷蛋白 磷酸化 侧纺状体 |
Enhancement of (-)-stepholidine on protein phosphorylation of a dopamine- and cAMP-regulated phosphoprotein in denervated striatum of oxidopamine-lesioned rats. |
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| X Guo,J Liu,L L Zou,J Jin,B C Wang,G Z Jin.Enhancement of (-)-stepholidine on protein phosphorylation of a dopamine- and cAMP-regulated phosphoprotein in denervated striatum of oxidopamine-lesioned rats.[J].Acta Pharmacologica Sinica,1998,19(2):100-103. |
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| Authors: | X Guo J Liu L L Zou J Jin B C Wang G Z Jin |
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| Institution: | Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China. |
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| Abstract: | AIM: To study effects of (-)-stepholidine (SPD) on the phosphorylation of a dopamine- and cAMP-regulated phosphoprotein (DARPP-32) in the striatum of oxidopamine-lesioned rats. METHODS: The amount of dephospho-DARPP-32 was measured by a back-phosphorylation assay. RESULTS: In the striatum of control rats, SPD per se had no effect on the phosphorylation of DARPP-32, but it antagonized the decrease by 28% of dephospho-DARPP-32 induced by the D1 agonist SK&F-38393. In the denervated striatum of oxidopamine-lesioned rats, SPD decreased the amount of dephospho-DARPP-32 by 44%. The effect of SPD was completely counteracted by the concomitant administration of the D1 antagonist Sch-23390. CONCLUSION: SPD exhibits D1 agonistic action on DARPP-32 phosphorylation in the denervated striatum of oxidopamine-lesioned rats, but it acts as a D1 antagonist in normal striatum. |
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