健脾补肾方对胃癌细胞系MGC-803线粒体膜电位和细胞内活性氧的影响

目的：研究健脾补肾方对MGC．803细胞凋亡的诱导作用及对线粒体膜电位和细胞内活性氧水平的影响。方法：应用流式细胞仪检测健脾补肾方含药血清诱导的MGC．803细胞凋亡及其线粒体膜电位和细胞内活性氧水平（分别用Annexin V、rhodamine 123和dihydrorhodamine 123）。结果：药物作用36h后，各用药组的细胞凋亡率显著高于空白血清组，药物作用有明显的时。效关系（P〈0．05）；药物作用36h后，中药组的细胞内活性氧水平基本稳定，而中西药组低于空白血清组和中药组（P〈0，05）；各用药组的线粒体膜电位水平随药物作用时间延长逐渐降低。结论：健脾补肾方可时间依赖地诱导MGC-803细胞凋亡，并可降低其线粒体膜电位，稳定细胞内活性氧水平；而且健脾补肾方与DDP具有协同作用。

Effect of Jianpi Bushen Formulation on the Level of Mitochondrial Membrane Potential and Endocellular Reactive Oxygen Species in MGC-803 Cells

Objective:To study the inducing effect of Jianpi Bushen Formulation(JPBS)on apoptosis,the levels of mitochondrial membrane potential(MMP)and endocellular reactive oxygen species(ROS)in MGC-803 cells.Methods:Apoptosis induced by JPBS-containing serum.MMP and ROS in MGC-803 cells was detected using flow cytometry(FCM)(Annexin V,rhodamine 123 and dihydrorhodamine 123 were used,respectively).Results:After 36 hours of treatment,the apoptosis of the drug-treated groups was obviously higher than that of the control group,and the drug action showed significant time-effect relationhip (P<0.05);The endocellular ROS level of JPBS group maintained substantially constant,and the level of JPBS+DDP group was lower than that of the serum control group and JPBS group(P<0.05)after 36 hours of treatment;and the MMP level of JPBS group,DDP group and JPBS+DDP group reduced gradually with extended action duration.Conclusion:Apoptosis induced by JPBS in MGC-803 cells may show time-dependent,the MMP level may be reduced and the endocellular ROS level may keep constant;and,there were synergetic effect between JPBS and DDR.