| 转化牛蒡子苷牛蒡内生菌的分离及L2菌株的鉴定 |
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| 引用本文： | 张淼,潘华奇,胡江春,窦德强,王书锦,康廷国.转化牛蒡子苷牛蒡内生菌的分离及L2菌株的鉴定[J].药物生物技术,2010(2). |
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| 作者姓名： | 张淼 潘华奇 胡江春 窦德强 王书锦 康廷国 |
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| 作者单位： | 辽宁中医药大学药学院;中国科学院沈阳应用生态研究所; |
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| 基金项目： | 国家十一五科技支撑计划项目(2006BAI09B20-6) |
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| 摘 要： | 从牛蒡(Arctium lappa L.)的根、茎、叶、总苞、种子中分离纯化了57株内生菌。以牛蒡子粉为底物,采用高效液相色谱法定量测定转化培养液中牛蒡子苷和牛蒡子苷元的含量,筛选得到6株转化牛蒡子苷的内生菌。其中L2的转化率最高,可达76.9%。利用LC-MS方法对其转化产物牛蒡子苷元进行了验证。L2经过形态观察、生理生化特征分析及16S rDNA序列分析,鉴定为氧化微杆菌(Microbacterium oxydans)。本试验结果为探索牛蒡内生菌转化牛蒡子苷成主要药效成分牛蒡子苷元技术提供了科学依据。
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| 关 键 词： | 牛蒡子苷 牛蒡子苷元 微生物转化 内生菌 鉴定 氧化微杆菌 |
Isoltion of Endophytic Microorganisms Transforming Arctiin from Arctium lappa L. and Identification of Bacterium L2 |
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| ZHANG Miao,PAN Hua-qi,HU Jiang-chun,DOU DE-qiang,WANG Shu-jin,KANG Ting-guo.Isoltion of Endophytic Microorganisms Transforming Arctiin from Arctium lappa L. and Identification of Bacterium L2[J].Pharmaceutical Biotechnology,2010(2). |
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| Authors: | ZHANG Miao PAN Hua-qi HU Jiang-chun DOU DE-qiang WANG Shu-jin KANG Ting-guo |
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| Institution: | ZHANG Miao1,2,PAN Hua-qi2,HU Jiang-chun1,DOU DE-qiang1,WANG Shu-jin2,KANG Ting-guo1(1.Liaoning University of Traditional Chinese Medicine,Dalian 116600,China,2.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China) |
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| Abstract: | To investigate the endophytic microorganisms to transform arctiin from Arctium lappa L.,endophytes were firstly isolated from surface-sterilized roots,stems,leaves,involucrum and seeds of Arctium lappa L.6 of the 57 endophytes obtained from A.lappa showed a promising potential to hydrolyse arctiin.Among them,strain L2 showed the highest transformation ratio(76.9%).Bacterium L2 was identified as Microbacterium oxydans L2 by a polyphasic approach including 16s rDNA sequencing and physiological and biochemical... |
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| Keywords: | Arctiin Arctigenin Biotransformation Endophytes Identification Microbacterium oxydans |
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