| 液相色谱-质谱联用法分析木瓜中绿原酸与抗癌活性成分的相关性研究 |
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| 引用本文： | 赵 光,李 珺,杜雪静.液相色谱-质谱联用法分析木瓜中绿原酸与抗癌活性成分的相关性研究[J].中国医学装备,2014(5):4-6. |
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| 作者姓名： | 赵 光 李 珺 杜雪静 |
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| 作者单位： | 首都医科大学化学生物学与药学院,北京100069 |
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| 基金项目： | 国家自然科学基金（30500648）“以FAS为靶从元宝枫发展无耐药性的抗肿瘤药物”;北京市自然科学基金资助（7103168）“以介孔材料为载体从天然产物发展抗肿瘤靶向给药系统”;北京市教育委员会科技发展计划面上项目（KM201010025011）“从虎杖等天然产物中发展肿瘤化学预防药物”;首都中医药研究专项（ZM02）“医院制剂抗瘤丸治疗神经胶质瘤的作用机理研究” |
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| 摘 要： | 目的：高效液相色谱-质谱联用法（HPLC-MS）分析木瓜中绿原酸与抗癌活性成分的相关性。方法：采用HPLC-MS联用法,在Diamonsil C18色谱柱（4.6 mm×250 mm,5μm）,乙腈-0.05%磷酸水溶液梯度洗脱;流速为0.8 ml/min;检测波长为325 nm;柱温为25℃。分离测定木瓜中绿原酸的含量。结果：木瓜中绿原酸的含量为1.3 mg/g,相对标准偏差（RSD）为0.4%,线性关系良好。结论：木瓜中含有绿原酸,其主要成分与木瓜对脂肪酸合酶（FAS）的抑制及抗肿瘤作用有潜在的相关性。
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| 关 键 词： | 木瓜 高效液相色谱-质谱 绿原酸 |
Analysis on chlorogenic acid of chaenomeles speciosa and its correlations with anticancer by high performance liquid chromatography-mass spectrometry |
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| ZHAO Guang;LI Jun;DU Xue-jing.Analysis on chlorogenic acid of chaenomeles speciosa and its correlations with anticancer by high performance liquid chromatography-mass spectrometry[J].China Medical Equipment,2014(5):4-6. |
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| Authors: | ZHAO Guang;LI Jun;DU Xue-jing |
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| Institution: | ZHAO Guang;LI Jun;DU Xue-jing( School of Chemical Biology and Phamaceutical Sciences, Capital Medical University, Beijing 100069, China.) |
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| Abstract: | Objective： To analyze chlorogenic acid of Chaenomeles speciosa by high performance liquid chromatography-mass spectrometry. Methods： A method for determining chlorogenic acid of Chaenomeles speciosa by HPLC-MS is described. The operating conditions were Diamonsil C18column（5 μm, 4.6×250 mm） at room temperature, acetonitrile-0.05% phosphoric acid solution as mobile phase by step gradients elution at a flow of 0.8 ml/min and UV detection at 325 nm.Results： The content of chlorogenic acid of Chaenomeles speciosa is 1.3 mg/g（RSD 0.4%）,respectively. Conclusion： Chlorogenic acid was detected from Chaenomeles speciosa, which may be related to its inhibition of FAS and the potential role of anticancer. |
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| Keywords: | Chaenomeles speciosa HPLC-MS Chlorogenic acid |
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