p16基因突变及表达与喉癌的相关性研究

目的　研究ｐ１６ 基因在喉癌发生过程中的表达及在喉癌诊断中的应用价值。方法　采用特异合成引物对ｐ１６ 基因的有关外显子进行ＰＣＲ 扩增,结合单链构象多态性技术,检测２９ 例喉癌手术标本中ｐ１６ 基因的突变,同时应用免疫组织化学方法观察其ｐ１６ 蛋白表达情况。结果　１１／２９ 的喉癌组织有ｐ１６ 基因第一、第二外显子的突变（ Ｐ＜ ０ ．０５） ,其中９／１１ 发生于喉癌Ⅲ、Ⅳ期;７／９ 伴颈淋巴结转移有者第一、第二外显子突变（ Ｐ＜ ０ ．０５） 。免疫组织化学法结果显示临床Ⅲ、Ⅳ期喉癌ｐ１６ 蛋白表达明显低于Ⅰ、Ⅱ期（ Ｐ＜ ０ ．０５） ,伴颈淋巴结转移组ｐ１６ 蛋白表达明显低于无颈淋巴结转移组（ Ｐ＜ ０ ．０５） 。结论　ｐ１６ 基因突变与喉癌的临床病程进展有关,在喉癌的诊断、判断预后及指导治疗上有重要意义。

Study on Correlation between the Mutation and Expression of p16 Gene and Laryneal Cancer

Objective To study p16 expression in genesis of larygeal cancer and its value in the diagnosis of laryngeal cancer.Method The mutation of p16 gene in laryngeal cancer cells was determined by PCR SSCP method with silver staining technique.29 cases of specimens from primary laryngeal cancer were assayed by ABC immunohisto chemical staining method.Results By using PCR SSCP technique ,the mutations of exon 1 2 in p16 gene in 11/29 laryngeal carcinoma 7/9 patients complicated with neck lymph noeds metastasis,and 9/11 occurred on stage Ⅲ～Ⅳ.Conclusions The mutation of p16 gene is associated with the clinical progress of laryngeal cancer.P16 might be a reliable marker for diagnosis prognosis and treatment guide of laryngeal carcinoma.