The inhibitory effect of oxymatrine of hepatitis B virus in vitro

Objective To study the inhibitory effect of oxymatrine on hepatitis B virus (HBV) in vitro.Methods Microparticle enzyme immunoassay, bDNA signal amplification assay was used for determining secreted HBsAg/HBeAg in the culture medium, HBV DNA from core particles in the cytoplasm and MTT colorimetric assay was used to assay the oxymatrine cytotoxity.Results The inhibitory rates of HBsAg and HBeAg were 40.57% and 48.27% by oxymatrine at the concentration of 2000*!μg/ml. At 100-2000*!μg/ml, it can remarkably decrease the level of viral core associated-HBV DNA in the cytoplasm. No significant toxicity was shown in such concentrations.Conclusion Oxymatrine has a potential anti-HBV activity in vitro.