人源Dickkopf1原核表达产物对体外培养的黑素细胞的抑制作用

目的:观察人源Dickkopf1原核表达产物(DKK1蛋白)对体外培养的黑素细胞的抑制作用。方法:采用MTT法测定细胞增殖情况;光学显微镜观察细胞形态学变化;氢氧化钠裂解法测定黑素合成;多巴氧化法测定酪氨酸酶活性。结果:DKK1蛋白对黑素细胞的增殖有抑制作用,能使细胞数明显减少(P<0.05);光学显微镜观察到DKK1蛋白作用的黑素细胞胞体较大,形态略呈多角形,树突较粗短;并能使黑素合成显著下降(P<0.05);使酪氨酸酶活性显著减弱(P<0.05)。结论:DKK1蛋白能抑制黑素细胞增殖、黑素合成、酪氨酸酶活性,为DKK1蛋白应用于治疗色素增多性疾病奠定实验基础。

Inhibitory effects of product expressed via human DKK1 prokaryon on melanocytic proliferation, melanin synthesis and tyrosinase activity in vitro

Objective To investigate the effect of DKK1 protein on melanocytes,which is human and expressed by prokaryon expression system.Methods MTT method,NaOH-assay and DOPA-hydrocarbonylation method were employed to measure the proliferation,melanin synthesis,tyrosinase activity of melanocytes.The change of melanocyte cell morphology was observed via light microscope.Results DKK1 protein induced a mild effect on melanocytic proliferation(P<0.05),While it showed a statistically significant decrease in the melanin synthesis(P< 0.05),and tryosinase activity(P<0.05).The melanocyte affected by DKK1 protein showed cell body bigger,shape polygon,dendrite shorter and thicker.Conclusion DKK1 protein inhibits melanocytic proliferation,melanin synthesis and tryosinase activity,which may be the foundation that make it valid in clinical treatment of the pigmented skin diseases.