| 高效液相色谱法测定利咽颗粒中次野鸢尾黄素的含量 |
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| 引用本文： | 林方,林学锦.高效液相色谱法测定利咽颗粒中次野鸢尾黄素的含量[J].海峡药学,2012,24(9):60-62. |
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| 作者姓名： | 林方 林学锦 |
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| 作者单位： | 福建省福州市第二医院 福州350006 |
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| 摘 要： | 目的建立利咽颗粒中次野鸢尾黄素的含量测定方法。方法采用高效液相色谱法,色谱柱为Hypersil BDS C18柱(4.6×250mm,5μm),流动相为乙腈-0.2%磷酸溶液(36∶64),检测波长为266nm,流速为1.0mL.min-1,柱温为30℃。结果本法精密度高,稳定性好,次野鸢尾黄素浓度在1.4464~8.6784μg.mL-1范围内具有良好的线性关系(r=0.9999);平均加样回收率97.5%,RSD为1.11%。结论本方法简便,准确,重现性好,可作为该制剂的含量测定方法。
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| 关 键 词： | 利咽颗粒 次野鸢尾黄素 高效液相色谱法 |
Determination of irisflorentin in Liyan granules by HPLC |
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| LIN Fang
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LIN Xue-jin.Determination of irisflorentin in Liyan granules by HPLC[J].Strait Pharmaceutical Journal,2012,24(9):60-62. |
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| Authors: | LIN Fang LIN Xue-jin |
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| Institution: | (Fujian Fuzhou Second Hospital,Fuzhou 350007,China) |
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| Abstract: | OBJECTIVE To establish a method for determination of irisflorentin in Liyan granules.METHODS Using a HPLC method with Hypersil BDS C18 column（4.6×250mm,5μm）,the mobile phase was a acetonitrile-0.2%phosphoric acid（36：64）,the detection wavelength was 266nm,flow rate was 1.0mL·min-1,and column temperature was 30℃.RESULTS The precision and stability were fine,The standard curve of irisflorentin was rectilinear within the range of 1.4464～8.6784μg·mL-1,r=0.9999.The average recovery of irisflorentin was 97.5% with RSD of 1.11%.CONCLUSION The method is simple,accurate and reproducible,and can be used for the determination of irisflorentin. |
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| Keywords: | Liyan granules Irisflorentin HPLC |
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