桑色素对迟发型超敏反应的作用研究

目的 桑色素(morin)对迟发型超敏反应的影响机制.方法 通过致敏剂DNFB诱发DTH模型,检测morin对模型小鼠耳廓重量变化及肿胀程度的影响,并将致敏侧耳组织做冷冻切片HE染色,使用光学显微镜观察炎性细胞浸润程度的变化；酶联免疫吸附法检测小鼠血清IFN-γ含量.结果 对各组小鼠耳廓组织进行称重及厚度测量,发现morin (50 mg/ml)处理组显著抑制DTH炎症反应,鼠耳重量减轻、厚度变薄,差异有统计学意义(P＜0.05)；HE染色切片显示morin处理组鼠耳水肿减轻,淋巴细胞等浸润程度显著减少；morin处理组DTH小鼠血清中IFN--γ的水平下降明显.结论 morin可能通过抑制淋巴细胞分泌1FN-γ水平,使DTH小鼠耳廓水肿和淋巴细胞浸润程度减轻.

The study of the effect of morin on delayed type hypersensitivity model of murine

Objective To discover the effects of morin on delayed type hypersensitivity model of murine.Methods DTH model was established and the effect of morin on DTH was evaluated,including the impact of morin on ear weight and thickness of DTH mice.Histological changes of mouse ear were observed under light microscopy,the levels of INF-γ in mouse serum were detected by ELISA.Results The results of ear weighting and thickness in three different groups(control group,DTH group and morin group) showed that morin significantly inhibited DTH reaction(P < 0.05).Observation of auricle tissue with H-E staining indicated morin markedly reduced the lymphocytes infiltration.50mg / ml morin also significant decreased the level of INF-γ in the serum of the DTH mice(P < 0.05).Conclusion The reduced INF-γ Production in lymphocytes may be one of mechanisms of morin suppress swelling and lymphocytes infiltration of the DTH mice.