A Therapeutic Concentrate of Coagulation Factors II, IX and X from Citrated, Factor VIII-Depleted Plasma |
| |
| Authors: | Sarah M. Middleton Ida H. Bennett and J. K. Smith |
| |
| Affiliation: | Scottish National Blood Transfusion Association, Protein Fractionation Centre, Royal Infirmary, Edinburgh |
| |
| Abstract: | Abstract. A simple procedure is described for the large-scale absorption on to DEAE-cellulose of coagulation factors II, IX and X from citrated, factor VIII-depleted plasma. The coagulation factors are eluted frontally from the exchanger in a high yield and in a form suitable for therapeutic use, without further fractionation. The lyophilised concentrate is very stable without the addition of heparin and, when redissolved to isoosmolar solution, contains approximately 30 U/ml factors II, IX and X, 250–300 times purified from the starting plasma. The effectiveness of the concentrate in the treatment of haemophilia B is discussed. |
| |
| Keywords: | |
|
|
