男性不育患者的细胞遗传学分析

目的对男性不育患者进行染色体核型的检测，以指导其生育。方法1．应用规范的WHO精液检查方法检查男性不育患者精液标本。2．应用细胞遗传学检测男性不育患者外周血染色体核型分析。结果1．精液检查结果：男性不育组患者356例，包括无精子症82例、严重少精子症126例、少弱精子症148例，精液正常、核型正常男性组对照50例。2．细胞染色体检查：356例男性不育患者全部进行外周血淋巴细胞染色体核型分析，发现55例染色体核型异常，包括性染色体异常24例，常染色体异常21例和染色体多态性10例。其中，无精子症组的染色体异常率25．61％（21／81），严重少精子症患者染色体核型异常率为11．90％（15／126），少弱精子症患者染色体核型异常率为12．84％（19／148）。结论男性不育患者核型异常率为15．45％，远高于正常人群发病率。细胞遗传学染色体检查对男性不育的生育指导有重要意义。无精子症患者进行染色体检查尤其必要。

Cytogenetic Analysis of infertile males

Objective： To analyze the karyotypes of infertile males so as to direct their procreation. Methods： 1. The semen of infertile males was detected by the WHO semen detection system. 2. Peripheral blood of 356 infertile males was gained, then we cultured lymphocytes, made slides and analyzed the karyotypes by G banding （C banding if necessary）. Results ： 1. Among 356 infertile men, there were 82 patients with azoospermia, 126 patients with severe oligospermia. 2. 55 cases were found with abnormal karyotypes in the 356 infertility cases （15. 45% ）, including 24 sex chromosome abnormality, 21 autosome abnormality and 10 polymorphism. The abnormal karyotype rate in azoospermia is 25.61% （21/81） and the rate in severe oligospermia is 11.90% （ 15/126）, while in oligospermia is 12. 84% （19/148）. Conclusions： The frequency of the chromosome abnormality in infertility males （ 15.45% ） is higher than the normal people. Our findings suggest that abnormal karyotypes can cause male infertility. Karyotype analysis is important in azoospermia people.