结肠癌患者外周血VEGF-C和CK20 mRNA的表达与淋巴转移的关系

目的探讨结肠癌患者外周血VEGF-C mRNA和CK20 mRNA的表达与淋巴转移的关系。方法应用RT-PCR检测80例结肠癌患者、30例结肠良性肿瘤患者、30例健康人外周血VEGF-C mRNA和CK20 mRNA的表达。结果 VEGF-C mRNA在结肠癌患者外周血的阳性表达率为52.5%,在结肠良性肿瘤、健康人的表达率分别为10.0%、0,其中有淋巴结转移的结肠癌患者的阳性表达明显高于无淋巴结转移的阳性表达(P<0.05)。CK20 mRNA在结肠癌患者外周血的阳性表达率为60.0%,在结肠良性肿瘤、健康人的表达率均为0,其中有淋巴结转移的结肠癌患者的阳性表达明显高于无淋巴结转移的阳性表达(P<0.05)。结论结肠癌患者外周血VEGF-C mRNA和CK20 mRNA的表达与淋巴转移相关。

The relationship of VEGF-C and CK20 mRNA expression in peripheral blood and lymphatic metastasis in patients with colon carcinoma

Objective To investigate the relationship of VEGF-C and CK20 mRNA expression in peripheral blood and lymphatic metastasis in patients with colon carcinoma.Methods RT-PCR was used to detect the expression of VEGF-C and CK20 mRNA in peripheral blood of 80 patients with colon carcinoma,30 patients with colon benign tumor and 30 healthy volunteers.Results The positive rate of VEGF-C mRNA in peripheral blood of patients with colon carcinoma was 52.5%,but 10% and 0 in colon benign tumor and healthy controls.The positive rate of CK20 mRNA in peripheral blood of patients with colon carcinoma was 60%,but 0 in colon benign tumor and healthy controls.The positive rate of CK20 and VEGF-C mRNA expressions with lymph node metastasis was significant higher than that without lymph node metastasis(P<0.05).Conclusion The expression of VEGF-C mRNA and CK20 mRNA in peripheral blood of patients with colon carcinoma are correlated with lymph node metastasis.